Gibco

Mouse TNF-alpha, Animal-Free Recombinant Protein, PeproTech®

产品信息

AF-315-01A-20UG

应用

建议稀释比

已发表文章

功能检测 (Functional)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

体外检测 (IV)

查看8篇已发表文章 8篇已发表文章

其他PubMed (Misc)

查看3篇已发表文章 3篇已发表文章

产品规格

种属

Mouse

已发表种属

Human, Mouse

Expression System

E. coli

氨基酸序列

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

分子量

17.3 kDa

分类

Recombinant

类型

Protein

纯度

≥ 98% by SDS-PAGE gel and HPLC analyses.

内毒素浓度

<0.1 EU/µg

活性

Determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

purified

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

产品详细信息

Recombinant Murine TNF-alpha is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Obeticholic acid improved triptolide/lipopolysaccharide-induced hepatotoxicity by inhibiting caspase-11-GSDMD pyroptosis pathway.

Journal of applied toxicology : JAT

Liang P,Zhou S,Yuan Z,Zhang L,Jiang Z,Yu Q

AF-315-01A was used in In vitro experiments to indicate that FXR might be an attractive therapeutic target for TP/LPS-induced hepatotoxicity, providing an effective strategy for drug-induced liver injury.

Sat Apr 01 00:00:00 UTC 2023

PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation.

Nature communications

Linnerbauer M,Beyer T,Nirschl L,Farrenkopf D,Lößlein L,Vandrey O,Peter A,Tsaktanis T,Kebir H,Laplaud D,Oellinger R,Engleitner T,Alvarez JI,Rad R,Korn T,Hemmer B,Quintana FJ,Rothhammer V

AF-315-01A was used in Cell Culture to suggest the glial PD-L1/PD-1 axis as a potential therapeutic target for both acute and progressive MS stages.

Sat Sep 09 00:00:00 UTC 2023

Bclaf1 regulates c-FLIP expression and protects cells from TNF-induced apoptosis and tissue injury.

EMBO reports

Zhang R,Xue T,Shao A,Lang Y,Qin C,Zhao M,Kuang Y,Yu Z,Geng Y,Zhao C,Tang J

AF-315-01A was used in In vivo experiments to suggest Bclaf1 is a key regulator in TNF-induced apoptosis, both in vitro and in vivo.

Wed Jan 05 00:00:00 UTC 2022

Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice.

Bioscience trends

Zhou Y,Cheng Y,Li Y,Ma J,Wu Z,Chen Y,Mei J,Chen M

AF-315-01A was used in Sample Prep (WB) to explore the relation of GABAergic transmission and soluble Aβ in early APP/PS1 mice.

Sun Nov 21 00:00:00 UTC 2021

CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype.

Journal of cellular and molecular medicine

Xie C,Ye F,Zhang N,Huang Y,Pan Y,Xie X

AF-315-01A was used in In vitro experiments to investigate chemokine activation of inflammation in abdominal aortic aneurysm.

Sun Aug 01 00:00:00 UTC 2021

MiR-1290 promotes myoblast differentiation and protects against myotube atrophy via Akt/p70/FoxO3 pathway regulation.

Skeletal muscle

Che J,Xu C,Wu Y,Jia P,Han Q,Ma Y,Wang X,Zheng Y

AF-315-01A was used in In vitro experiments to investigate miRNA-1290 in sarcopenia.

Mon Mar 15 00:00:00 UTC 2021

Role and mechanism underlying FoxO6 in skeletal muscle in vitro and in vivo.

International journal of molecular medicine

Zhang L,Zhang Y,Zhou M,Wang S,Li T,Hu Z,Jin C

AF-315-01A was used in In vitro experiments to suggest that FoxO6 served a critical role in muscle cell metabolism in vitro and in vivo, and might serve as a promising therapeutic target for ameliorating skeletal muscle atrophy.

Thu Jul 01 00:00:00 UTC 2021

Dietary tryptophan links encephalogenicity of autoreactive T cells with gut microbial ecology.

Nature communications

Sonner JK,Keil M,Falk-Paulsen M,Mishra N,Rehman A,Kramer M,Deumelandt K,Röwe J,Sanghvi K,Wolf L,von Landenberg A,Wolff H,Bharti R,Oezen I,Lanz TV,Wanke F,Tang Y,Brandao I,Mohapatra SR,Epping L,Grill A,Röth R,Niesler B,Meuth SG,Opitz CA,Okun JG,Reinhardt C,Kurschus FC,Wick W,Bode HB,Rosenstiel P,Platten M

Fri Oct 25 00:00:00 UTC 2019

Silencing of PTGS2 exerts promoting effects on angiogenesis endothelial progenitor cells in mice with ischemic stroke via repression of the NF-κB signaling pathway.

Journal of cellular physiology

Zhou Z,Lu C,Meng S,Dun L,Yin N,An H,Xu H,Liu G,Cai Y

AF-315-01A was used in In vitro experiments to conclude that the specific knockdown of PTGS2 expression could repress the NF-κB signaling pathway, thereby inhibits apoptosis and promotes proliferation, migration and angiogenesis of EPCs, providing protective effect on mice with ischemic stroke.

Sun Dec 01 00:00:00 UTC 2019

Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock.

Immunity

Mandal P,Feng Y,Lyons JD,Berger SB,Otani S,DeLaney A,Tharp GK,Maner-Smith K,Burd EM,Schaeffer M,Hoffman S,Capriotti C,Roback L,Young CB,Liang Z,Ortlund EA,DiPaolo NC,Bosinger S,Bertin J,Gough PJ,Brodsky IE,Coopersmith CM,Shayakhmetov DM,Mocarski ES

Tue Jul 17 00:00:00 UTC 2018

CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata.

Journal of immunology (Baltimore, Md. : 1950)

Dai Z,Xing L,Cerise J,Wang EH,Jabbari A,de Jong A,Petukhova L,Christiano AM,Clynes R

Mon Aug 15 00:00:00 UTC 2016

Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity.

Scientific reports

Pepin M,Mezouar S,Pegon J,Muczynski V,Adam F,Bianchini EP,Bazaa A,Proulle V,Rupin A,Paysant J,Panicot-Dubois L,Christophe OD,Dubois C,Lenting PJ,Denis CV

Mon Nov 28 00:00:00 UTC 2016

生物信息学

蛋白别名: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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基因别名: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

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UniProt ID: (Mouse) P06804

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Entrez Gene ID: (Mouse) 21926

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Mouse TNF-alpha, Animal-Free Recombinant Protein, PeproTech®

产品信息

AF-315-01A-20UG

功能检测 (Functional)

体外检测 (IV)

其他PubMed (Misc)

种属

已发表种属

Expression System

氨基酸序列

分子量

分类

类型

纯度

内毒素浓度

活性

偶联物

形式

纯化类型

内含物

保存条件

运输条件

产品详细信息

靶标信息

生物信息学

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