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产品信息
PRC7015
应用
建议稀释比
已发表文章
产品规格
种属
Rat
Expression System
E. coli
氨基酸序列
Rat CNTF recombinant protein contains 199 amino acids
分子量
22.7 kDa
分类
Recombinant
类型
Protein
纯度
≥98% by SDS-PAGE and HPLC
内毒素浓度
<0.1 ng/µg
活性
ED50 = 25 - 35 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
5mM sodium acetate, pH 6.5
内含物
no preservative
保存条件
-20°C
运输条件
Wet ice (domestic); Wet or Dry ice (international)
产品详细信息
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute recombinant rat CNTF in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
靶标信息
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
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生物信息学
蛋白别名: Ciliary neurotrophic factor; ciliary neurotropic factor; CNTF; H-CNTF
基因别名: Cntf
UniProt ID: (Rat) P20294
Entrez Gene ID: (Rat) 25707
