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Invitrogen
SARS-CoV-2 Spike Protein S1/S2 (BA.4) His-tag, trimer Recombinant Protein
产品信息
RP-88549
产品规格
种属
Virus
Expression System
HEK293 cells
氨基酸序列
SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1) with variant BA.4 mutations expressed with the bacteriophage T4 fibritin and a C-terminal His-tag. Full sequence information available in Product Specfic Information section.
标签
His-tag
分子量
136.26 kDa
分类
Recombinant
类型
Protein
纯度
>90% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
内毒素浓度
<1.0 EU/µg
活性
Immobilized human ACE2 protein (mFc tag) at 2 µg/mL (100 µL/well) can bind Recombinant SARS-CoV-2 (BA.4) Spike S1+S2 trimer Protein (ECD, His Tag), the EC50 is 8-32 ng/mL.
偶联物
形式
Lyophilized
浓度
0.25 mg/mL
纯化类型
purified
保存液
25mM sodium citrate, pH 6, with 5% mannitol, 200mM NaCl, 5% trehalose, 0.01% Tween 80
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 uL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1) with (LPL24-26, HV69-70) deletions, and F817P, A892P, A899P, A942P, K986P, V987P, T19I, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K substitutions and furin cleavage site mutations expressed with the bacteriophage T4 fibritin and a C-terminal His-tag.
The mutations were identified in the SARS-CoV-2 varient known as BA.4 or BA.5 or BA.5.2.
靶标信息
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2
