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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 506, eBioscience™
图: 1 / 1
Rat IgG1 kappa Isotype Control (69-4301-81) in Flow
C57Bl/6 mouse splenocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and staining buffer (autofluorescence, left) or 0.125 µg of Rat IgG1 kappa Isotype Control, eFluor 506 (right). Total viable cells were used for analysis.
产品信息
69-4301-81
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
产品规格
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eBRG1
偶联物
激发/发射光谱
419/508 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2784890
产品详细信息
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis.
Applications Tested: The eFluor 506 rat IgG1 isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at the same concentration as the experimental antibody.
eFluor 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
靶标信息
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
