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B7-H6 Antibody (12-6526-42) in Flow
HeLa cells were stained with Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (blue) or B7-H6 Monoclonal Antibody, PE (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), were used for analysis.
产品信息
12-6526-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
JAM1EW
抗原
B7-H6 recombinant protein
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2784629
产品详细信息
Description: This JAM1EW monoclonal antibody recognizes human B7-H6 (NCR3LG1) protein on the surface of cells. It recognizes protein on live but not on paraformaldehyde-fixed cells. The JAM1EW clone is specific to B7-H6. When tested in flow cytometry against cells transfected with either B7-H4, B7-H5, B7-H6, or B7-H7, clone JAM1EW only stains positively on B7-H6 transfected cells.
Applications Reported: This JAM1EW antibody has been reported for use in flow cytometric analysis.
Applications Tested: This JAM1EW antibody has been pre-diluted and tested by flow cytometric analysis of HeLa and HL-60 cell line. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
靶标信息
B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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生物信息学
蛋白别名: B7 homolog 6; B7-H6; DKFZp686I21167; Natural cytotoxicity triggering receptor 3 ligand 1; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
基因别名: B7-H6; B7H6; DKFZp686O24166; NCR3LG1
UniProt ID: (Human) Q68D85
Entrez Gene ID: (Human) 374383
