Invitrogen

CD103 (Integrin alpha E) Monoclonal Antibody (2E7), eFluor™ 450, eBioscience™

CD103 (Integrin alpha E) Antibody in Flow Cytometry (Flow)

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CD103 (Integrin alpha E) Antibody (48-1031-82) in Flow

Staining of C57Bl/6 lymph node cells with Anti-Mouse CD3e APC (Product # 17-0031-82) and 1.0 µg of Armenian Hamster IgG Isotype Control eFluor® 450 (Product # 48-4888-82) (left) or 1.0 µg of Anti-Mouse CD103 (Integrin alpha E) eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

CD103 (Integrin alpha E) Antibody in Immunohistochemistry (IHC)

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产品信息

48-1031-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看19篇已发表文章 19篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2E7

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574033

产品详细信息

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2E7 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes.

iScience

Spath S,Roan F,Presnell SR,Höllbacher B,Ziegler SF

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Fri Sep 16 00:00:00 UTC 2022

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Mon Jan 24 00:00:00 UTC 2022

Macrophages Maintain Epithelium Integrity by Limiting Fungal Product Absorption.

Cell

Chikina AS,Nadalin F,Maurin M,San-Roman M,Thomas-Bonafos T,Li XV,Lameiras S,Baulande S,Henri S,Malissen B,Lacerda Mariano L,Barbazan J,Blander JM,Iliev ID,Matic Vignjevic D,Lennon-Duménil AM

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Thu Oct 15 00:00:00 UTC 2020

Club Cell TRPV4 Serves as a Damage Sensor Driving Lung Allergic Inflammation.

Cell host & microbe

Wiesner DL,Merkhofer RM,Ober C,Kujoth GC,Niu M,Keller NP,Gern JE,Brockman-Schneider RA,Evans MD,Jackson DJ,Warner T,Jarjour NN,Esnault SJ,Feldman MB,Freeman M,Mou H,Vyas JM,Klein BS

48-1031 was used in Flow cytometry/Cell sorting to conclude that club cells detect junction damage as mechanical stress, which signals danger via TRPV4, calcium, and calcineurin to initiate allergic sensitization.

Wed Apr 08 00:00:00 UTC 2020

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Sat Feb 01 00:00:00 UTC 2020

Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer.

Therapeutic advances in medical oncology

Doo DW,Meza-Perez S,Londoño AI,Goldsberry WN,Katre AA,Boone JD,Moore DJ,Hudson CT,Betella I,McCaw TR,Gangrade A,Bao R,Luke JJ,Yang ES,Birrer MJ,Starenki D,Cooper SJ,Buchsbaum DJ,Norian LA,Randall TD,Arend RC

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Thu Apr 14 00:00:00 UTC 2022

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Tue Nov 03 00:00:00 UTC 2020

Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy.

Nature communications

Rosato PC,Wijeyesinghe S,Stolley JM,Nelson CE,Davis RL,Manlove LS,Pennell CA,Blazar BR,Chen CC,Geller MA,Vezys V,Masopust D

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Mon Feb 04 00:00:00 UTC 2019

CSF1R antagonism limits local restimulation of antiviral CD8⁺ T cells during viral encephalitis.

Journal of neuroinflammation

Funk KE,Klein RS

48-1031 was used in Flow cytometry/Cell sorting to highlight the impact of colony-stimulating factor 1 receptor antagonism on APC activation in the CNS and periphery and the importance of microglia in orchestrating the CNS immune response following neurotropic viral infection.

Thu Jan 31 00:00:00 UTC 2019

Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3⁺RORγt⁺ Regulatory T Cells.

Cell reports

Ogawa C,Bankoti R,Nguyen T,Hassanzadeh-Kiabi N,Nadeau S,Porritt RA,Couse M,Fan X,Dhall D,Eberl G,Ohnmacht C,Martins GA

48-1031-82 was used in Flow Cytometry to study the role of Blimp-1 as a crucial regulator of the Foxp3+RORγt+ regulatory T cell subset.

Tue Oct 02 00:00:00 UTC 2018

NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth.

Nature immunology

Chen L,Wilson JE,Koenigsknecht MJ,Chou WC,Montgomery SA,Truax AD,Brickey WJ,Packey CD,Maharshak N,Matsushima GK,Plevy SE,Young VB,Sartor RB,Ting JP

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

48-1031 was used in Flow cytometry/Cell sorting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 UTC 2017

Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.

The EMBO journal

Kawasaki T,Ito K,Miyata H,Akira S,Kawai T

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Wed Jun 14 00:00:00 UTC 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Immunofluorescence

Wed Apr 05 00:00:00 UTC 2017

Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome.

Cell

Chudnovskiy A,Mortha A,Kana V,Kennard A,Ramirez JD,Rahman A,Remark R,Mogno I,Ng R,Gnjatic S,Amir ED,Solovyov A,Greenbaum B,Clemente J,Faith J,Belkaid Y,Grigg ME,Merad M

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Thu Oct 06 00:00:00 UTC 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Mon Aug 15 00:00:00 UTC 2016

Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine.

Scientific reports

Ochi T,Feng Y,Kitamoto S,Nagao-Kitamoto H,Kuffa P,Atarashi K,Honda K,Teitelbaum DH,Kamada N

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 48-1031-82) in Flow Cytometry

Wed Jun 15 00:00:00 UTC 2016

Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection.

PLoS pathogens

Wiesner DL,Specht CA,Lee CK,Smith KD,Mukaremera L,Lee ST,Lee CG,Elias JA,Nielsen JN,Boulware DR,Bohjanen PR,Jenkins MK,Levitz SM,Nielsen K

48-1031 was used in Flow cytometry/Cell sorting to investigate Th2 cell responses to pulmonary fungal infection, showing that chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection.