Invitrogen

CD150 Monoclonal Antibody (mShad150), PerCP-eFluor™ 710, eBioscience™

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CD150 Antibody (46-1502-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.06 µg of Anti-Mouse CD150 PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

Product Image Gallery

CD150 Antibody — Fig 1 YH250 stimulate hematopoiesis recovery from 7Gy radiation. (A) Bone marrow cells from either YH250 or DMSO treated 7Gy irradiated animals were recovered and FACS analyzed, there are more Lin - CD48 - CD150 + cells in YH250 treated animals. (B) At different time post radiation, bone marrow cells were recovered for competitive repopulation or (D) CFC assays. (C) Bone marrow cells recovered at day 14 post radiation from YH250 treated animals give significantly better long-term engraftment in competitive repopulation and (E) more colonies in CFC assay. {TM}

产品信息

46-1502-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, lambda

分类

Monoclonal

类型

Antibody

克隆号

mShad150

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2016699

产品详细信息

Description: The mShad150 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells, in particular Th1, and B cells; this expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. The mShad150 antibody has been reported to also stain the CD150+CD244-CD48-CD41- population of pluripotent hematopoietic stem cells.

Applications Reported: This mShad150 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This mShad150 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Bone marrow adipocytes drive the development of tissue invasive Ly6C^high monocytes during obesity.

eLife

Boroumand P,Prescott DC,Mukherjee T,Bilan PJ,Wong M,Shen J,Tattoli I,Zhou Y,Li A,Sivasubramaniyam T,Shi N,Zhu LY,Liu Z,Robbins C,Philpott DJ,Girardin SE,Klip A

46-1502-82 was used in Flow Cytometry to find a temporal and causal link between BM adipocyte whitening and the Ly6Chigh monocyte surge, preceding the adipose tissue macrophage rise during HFD in mice.

Tue Sep 20 00:00:00 UTC 2022

Loss of tRNA-modifying enzyme Elp3 activates a p53-dependent antitumor checkpoint in hematopoiesis.

The Journal of experimental medicine

Rosu A,El Hachem N,Rapino F,Rouault-Pierre K,Jorssen J,Somja J,Ramery E,Thiry M,Nguyen L,Jacquemyn M,Daelemans D,Adams CM,Bonnet D,Chariot A,Close P,Bureau F,Desmet CJ

46-1502-82 was used in flow cytometry to investigate Elp3 and p53 in hematopoiesis.

Mon Mar 01 00:00:00 UTC 2021

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

Published figure using CD150 monoclonal antibody (Product # 46-1502-82) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2022

Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.

Journal of neuroinflammation

Hohsfield LA,Najafi AR,Ghorbanian Y,Soni N,Hingco EE,Kim SJ,Jue AD,Swarup V,Inlay MA,Green KN

46-1502 was used in Flow Cytometry to characterize the role and complexity of myeloid cells in the regulation of CNS cells and functional outcomes in the brain.

Sun Sep 20 00:00:00 UTC 2020

Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.

Journal of neuroinflammation

Hohsfield LA,Najafi AR,Ghorbanian Y,Soni N,Hingco EE,Kim SJ,Jue AD,Swarup V,Inlay MA,Green KN

46-1502 was used in Flow Cytometry to characterize the role and complexity of myeloid cells in the regulation of CNS cells and functional outcomes in the brain.

Sun Sep 20 00:00:00 UTC 2020

Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting.

Cancer cell

Srivastava S,Salter AI,Liggitt D,Yechan-Gunja S,Sarvothama M,Cooper K,Smythe KS,Dudakov JA,Pierce RH,Rader C,Riddell SR

46-1502 was used in Flow cytometry/Cell sorting to demonstrate the use of “AND” logic gates as a strategy to increase the portfolio of cell surface molecules on solid tumors that can be targeted safely with CAR-T cells.

Mon Mar 18 00:00:00 UTC 2019

c-Myb Exacerbates Atherosclerosis through Regulation of Protective IgM-Producing Antibody-Secreting Cells.

Cell reports

Shikatani EA,Besla R,Ensan S,Upadhye A,Khyzha N,Li A,Emoto T,Chiu F,Degousee N,Moreau JM,Perry HM,Thayaparan D,Cheng HS,Pacheco S,Smyth D,Noyan H,Zavitz CCJ,Bauer CMT,Hilgendorf I,Libby P,Swirski FK,Gommerman JL,Fish JE,Stampfli MR,Cybulsky MI,Rubin BB,Paige CJ,Bender TP,McNamara CA,Husain M,Robbins CS