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CD162 (PSGL-1) Antibody (16-1621-85) in ICC/IF, Flow
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 -
Figure 3 Macrophage targeting ability and cellular internalization of PLM-miRs in vitro. A) The binding ability of DiD-labeled LM-miRs or PLM-miRs to inflamed (or not) BMDMs was detected by flow cytometry and C) statistically analyzed by FlowJo_V10 software ( n = 3). B) The binding of LM-miRs, PLM-miRs, and anti-P-selectin-blocked PLM-miRs to inflamed (or not) BMDMs were imaged after staining with PSGL-1 an... View More
产品信息
16-1621-85
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
4RA10
抗原
Mouse PSGL-1 human IgG1 fusion protein
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2848284
产品详细信息
Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L.
Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis. (Fluorochrome-conjugated 4RA10 is recommended for use in flow cytometry).
Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand-1; selectin P ligand; SELPG
基因别名: CD162; Psgl-1; Psgl1; Selp1; Selpl
Entrez Gene ID: (Mouse) 20345
