Invitrogen

CD202b (TIE2) Monoclonal Antibody (TEK4), eBioscience™

CD202b (TIE2) Antibody in Flow Cytometry (Flow)

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CD202b (TIE2) Antibody (14-5987-82) in Flow

Staining of mouse bone marrow with Anti-Mouse CD202b (TIE2) PE and Anti-Mouse Ly-6A/E (Sca-1) APC (Product # 17-5981-82) + Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82). Total viable lineage negative cells were used for analysis.

CD202b (TIE2) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD202b (TIE2) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD202b (TIE2) Antibody in Immunocytochemistry (ICC/IF)

CD202b (TIE2) Antibody in Immunohistochemistry (IHC)

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产品信息

14-5987-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

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免疫细胞化学 (ICC/IF)

查看5篇已发表文章 5篇已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse, Pig

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

TEK4

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_467792

产品详细信息

Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.

Applications Reported: This TEK4 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Pulmonary Endothelial Glycocalyx Modifications in Glypican 1 Knockout Mice Do Not Affect Lung Endothelial Function in Physiological Conditions.

International journal of molecular sciences

Thota LNR,Lopez Rosales JE,Placencia I,Zemskov EA,Tonino P,Michael AN,Black SM,Chignalia AZ

14-5987-82 was used in Immunocytochemistry to indicate that the deletion of glypican 1 may result in the modification of the glycocalyx without affecting basal lung endothelial function, validating this mouse model as a tool for mechanistic studies that investigate the role of glypican 1 in lung endothelial function.

Tue Sep 26 00:00:00 UTC 2023

Distinct skeletal stem cell types orchestrate long bone skeletogenesis.

eLife

Ambrosi TH,Sinha R,Steininger HM,Hoover MY,Murphy MP,Koepke LS,Wang Y,Lu WJ,Morri M,Neff NF,Weissman IL,Longaker MT,Chan CK

14-5987-82 was used in Flow Cytometry to address previously unappreciated shortcomings of SSC research.

Mon Jul 19 00:00:00 UTC 2021

ETV2-null porcine embryos survive to post-implantation following incomplete enucleation.

Reproduction (Cambridge, England)

Maeng G,Gong W,Das S,Yannopoulos D,Garry DJ,Garry MG

14-5987 was used in Immunohistochemistry to we report that the rate of complete enucleation (CE) using the blind method was 80.2 1.7%, although this significantly increased when the polar body-MII deviation was minimized (45°).

Fri May 01 00:00:00 UTC 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Fri May 08 00:00:00 UTC 2020

Elucidating the fundamental fibrotic processes driving abdominal adhesion formation.

Nature communications

Foster DS,Marshall CD,Gulati GS,Chinta MS,Nguyen A,Salhotra A,Jones RE,Burcham A,Lerbs T,Cui L,King ME,Titan AL,Ransom RC,Manjunath A,Hu MS,Blackshear CP,Mascharak S,Moore AL,Norton JA,Kin CJ,Shelton AA,Januszyk M,Gurtner GC,Wernig G,Longaker MT

14-5987 was used in Flow cytometry/Cell sorting to present a systematic analysis of mouse and human adhesion tissues.

Thu Aug 13 00:00:00 UTC 2020

Mesenchymal VEGFA induces aberrant differentiation in heterotopic ossification.

Bone research

Hwang C,Marini S,Huber AK,Stepien DM,Sorkin M,Loder S,Pagani CA,Li J,Visser ND,Vasquez K,Garada MA,Li S,Xu J,Hsu CY,Yu PB,James AW,Mishina Y,Agarwal S,Li J,Levi B

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Sun Oct 17 00:00:00 UTC 2021

Protumoral bone marrow-derived cells migrate via Gβγ-dependent signaling pathways and exhibit a complex repertoire of RhoGEFs.

Journal of cell communication and signaling

Cervantes-Villagrana RD,Color-Aparicio VM,Reyes-Cruz G,Vázquez-Prado J

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Sat Jun 01 00:00:00 UTC 2019

Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis.

The Journal of clinical investigation

La Porta S,Roth L,Singhal M,Mogler C,Spegg C,Schieb B,Qu X,Adams RH,Baldwin HS,Savant S,Augustin HG

14-5987 was used in Immunohistochemistry-immunofluorescence to define the regulatory functions of Tie1 during angiogenesis and vascular abnormalisation and identify its role during metastasis.

Thu Feb 01 00:00:00 UTC 2018

Isolation and functional assessment of mouse skeletal stem cell lineage.

Nature protocols

Gulati GS,Murphy MP,Marecic O,Lopez M,Brewer RE,Koepke LS,Manjunath A,Ransom RC,Salhotra A,Weissman IL,Longaker MT,Chan CKF

14-5987-82 was used in Flow Cytometry, Immunohistochemistry (Frozen) to describe a flow cytometry-based approach that does not require in vitro selection and that uses eight surface markers to distinguish and isolate mouse skeletal stem cells (mSSCs); bone, cartilage, and stromal progenitors (mBCSPs); and five downstream differentiated subtypes, including chondroprogenitors, two types of osteoprogenitors, and two types of hematopoiesis-supportive stroma.

Fri Jun 01 00:00:00 UTC 2018

Isolation and functional assessment of mouse skeletal stem cell lineage.

Nature protocols

Gulati GS,Murphy MP,Marecic O,Lopez M,Brewer RE,Koepke LS,Manjunath A,Ransom RC,Salhotra A,Weissman IL,Longaker MT,Chan CKF

Fri Jun 01 00:00:00 UTC 2018

Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism.

Science translational medicine

Karagiannis GS,Pastoriza JM,Wang Y,Harney AS,Entenberg D,Pignatelli J,Sharma VP,Xue EA,Cheng E,D'Alfonso TM,Jones JG,Anampa J,Rohan TE,Sparano JA,Condeelis JS,Oktay MH

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Immunohistochemistry

Wed Jul 05 00:00:00 UTC 2017

Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism.

Science translational medicine

Karagiannis GS,Pastoriza JM,Wang Y,Harney AS,Entenberg D,Pignatelli J,Sharma VP,Xue EA,Cheng E,D'Alfonso TM,Jones JG,Anampa J,Rohan TE,Sparano JA,Condeelis JS,Oktay MH

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Immunohistochemistry

Wed Jul 05 00:00:00 UTC 2017

The Selective Tie2 Inhibitor Rebastinib Blocks Recruitment and Function of Tie2^Hi Macrophages in Breast Cancer and Pancreatic Neuroendocrine Tumors.

Molecular cancer therapeutics

Harney AS,Karagiannis GS,Pignatelli J,Smith BD,Kadioglu E,Wise SC,Hood MM,Kaufman MD,Leary CB,Lu WP,Al-Ani G,Chen X,Entenberg D,Oktay MH,Wang Y,Chun L,De Palma M,Jones JG,Flynn DL,Condeelis JS

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Immunofluorescence

Wed Nov 01 00:00:00 UTC 2017

Pericyte-expressed Tie2 controls angiogenesis and vessel maturation.

Nature communications

Teichert M,Milde L,Holm A,Stanicek L,Gengenbacher N,Savant S,Ruckdeschel T,Hasanov Z,Srivastava K,Hu J,Hertel S,Bartol A,Schlereth K,Augustin HG

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Tue Jul 18 00:00:00 UTC 2017

Primitive erythrocytes are generated from hemogenic endothelial cells.

Scientific reports

Stefanska M,Batta K,Patel R,Florkowska M,Kouskoff V,Lacaud G

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Tue Jul 25 00:00:00 UTC 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation.

Developmental cell

Goode DK,Obier N,Vijayabaskar MS,Lie-A-Ling M,Lilly AJ,Hannah R,Lichtinger M,Batta K,Florkowska M,Patel R,Challinor M,Wallace K,Gilmour J,Assi SA,Cauchy P,Hoogenkamp M,Westhead DR,Lacaud G,Kouskoff V,Göttgens B,Bonifer C

Mon Mar 07 00:00:00 UTC 2016

Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation.

Developmental cell

Mon Mar 07 00:00:00 UTC 2016

ECM-Dependence of Endothelial Progenitor Cell Features.

Journal of cellular biochemistry

Siavashi V,Nassiri SM,Rahbarghazi R,Vafaei R,Sariri R

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

G-Protein-Coupled Receptor-2-Interacting Protein-1 Controls Stalk Cell Fate by Inhibiting Delta-like 4-Notch1 Signaling.

Cell reports

Majumder S,Zhu G,Xu X,Senchanthisai S,Jiang D,Liu H,Xue C,Wang X,Coia H,Cui Z,Smolock EM,Libby RT,Berk BC,Pang J

14-5987 was used in Immunohistochemistry to show that GIT1 inhibits the Notch1-Dll4 signaling pathway by competing with Notch1 ANK domain for binding to RBP-J in stalk cells.

Tue Dec 06 00:00:00 UTC 2016

The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.

The Journal of biological chemistry

Shi X,Zirbes KM,Rasmussen TL,Ferdous A,Garry MG,Koyano-Nakagawa N,Garry DJ

14-5987 was used in Immunohistochemistry to elucidate the role of Mesp1 and Creb1 in upstream transactivation of Ets variant 2 expression during embryogenesis.

Fri Apr 10 00:00:00 UTC 2015

The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.

The Journal of biological chemistry

Shi X,Zirbes KM,Rasmussen TL,Ferdous A,Garry MG,Koyano-Nakagawa N,Garry DJ

14-5987 was used in Immunohistochemistry to elucidate the role of Mesp1 and Creb1 in upstream transactivation of Ets variant 2 expression during embryogenesis.

Fri Apr 10 00:00:00 UTC 2015

Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis.

The Journal of clinical investigation

Moore-Morris T,Guimarães-Camboa N,Banerjee I,Zambon AC,Kisseleva T,Velayoudon A,Stallcup WB,Gu Y,Dalton ND,Cedenilla M,Gomez-Amaro R,Zhou B,Brenner DA,Peterson KL,Chen J,Evans SM

14-5987 was used in Immunofluorescence to study the the origins of cardiac fibroblasts during development and in fibrosis.

Tue Jul 01 00:00:00 UTC 2014

A crucial role for the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification.

Development (Cambridge, England)

Gilmour J,Assi SA,Jaegle U,Kulu D,van de Werken H,Clarke D,Westhead DR,Philipsen S,Bonifer C

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Sun Jun 01 00:00:00 UTC 2014

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Developmental biology

Shi X,Richard J,Zirbes KM,Gong W,Lin G,Kyba M,Thomson JA,Koyano-Nakagawa N,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Thu May 15 00:00:00 UTC 2014

The Wilms' tumour suppressor Wt1 is a major regulator of tumour angiogenesis and progression.

Nature communications

Wagner KD,Cherfils-Vicini J,Hosen N,Hohenstein P,Gilson E,Hastie ND,Michiels JF,Wagner N

14-5987 was used in Flow cytometry/Cell sorting to show that inducible knockout of Wt1 is sufficient to cause regression of tumour vascularisation and enhanced anti-tumour immune responses in mice.

Tue Dec 16 00:00:00 UTC 2014

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Immunofluorescence

Wed Aug 01 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Immunofluorescence

Wed Aug 01 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Immunofluorescence

Wed Aug 01 00:00:00 UTC 2012

Snail promotes the cell-autonomous generation of Flk1(+) endothelial cells through the repression of the microRNA-200 family.

Stem cells and development

Gill JG,Langer EM,Lindsley RC,Cai M,Murphy TL,Murphy KM

14-5987 was used in Flow cytometry/Cell sorting to assess the role of snail to promote the cell-autonomous induction of Flk1(+) endothelial cells in mouse embryonic stem cells.

Fri Jan 20 00:00:00 UTC 2012

ER71 directs mesodermal fate decisions during embryogenesis.

Development (Cambridge, England)

Rasmussen TL,Kweon J,Diekmann MA,Belema-Bedada F,Song Q,Bowlin K,Shi X,Ferdous A,Li T,Kyba M,Metzger JM,Koyano-Nakagawa N,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2011

ER71 directs mesodermal fate decisions during embryogenesis.

Development (Cambridge, England)

Rasmussen TL,Kweon J,Diekmann MA,Belema-Bedada F,Song Q,Bowlin K,Shi X,Ferdous A,Li T,Kyba M,Metzger JM,Koyano-Nakagawa N,Garry DJ

Published figure using CD202b (TIE2) monoclonal antibody (Product # 14-5987-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2011

Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos.

Development (Cambridge, England)

Yokomizo T,Dzierzak E

14-5987 was used in Immunohistochemistry to devise a method to make transparent whole mouse embryos for the observation of hematopoietic clusters.

Mon Nov 01 00:00:00 UTC 2010

Angiopoietin-2-driven vascular remodeling in airway inflammation.

The American journal of pathology

Tabruyn SP,Colton K,Morisada T,Fuxe J,Wiegand SJ,Thurston G,Coyle AJ,Connor J,McDonald DM

14-5987 was used in Immunohistochemistry to determine the precise contribution of Ang2 to the transformation of capillaries into venules.

Wed Dec 01 00:00:00 UTC 2010

Isolation of murine hair-inducing cells using the cell surface marker prominin-1/CD133.

The Journal of investigative dermatology

Ito Y,Hamazaki TS,Ohnuma K,Tamaki K,Asashima M,Okochi H

14-5987 was used in Flow cytometry/Cell sorting to propose that the CD133 is a novel surface marker useful for collecting DP cells in the anagen stage.

Tue May 01 00:00:00 UTC 2007

Mapping of angiogenic markers for targeting of vectors to tumor vascular endothelial cells.

Cancer gene therapy

Tang Y,Borgstrom P,Maynard J,Koziol J,Hu Z,Garen A,Deisseroth A

Sun Apr 01 00:00:00 UTC 2007

生物信息学

蛋白别名: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; HYK; mTIE2; p140 TEK; STK1; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

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基因别名: AA517024; Cd202b; Hyk; STK1; Tek; Tie-2; Tie2

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UniProt ID: (Mouse) Q02858

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Entrez Gene ID: (Mouse) 21687

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功能