Invitrogen

CD34 Monoclonal Antibody (581), PE-Cyanine5.5

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CD34 Antibody (CD34-581-18) in Flow

The Journal of experimental medicine 2015 - Figure 8. Distribution of hGMDPs, hMDPs, and hCDPs in adult hematopoietic organs. (a) Representative flow cytometry plots of gated CD45 + Lin(CD3/19/56/14) - CD34 + cells show hGMDPs, hMDPs, and hCDPs in human BM ( n = 4), peripheral blood (PBMC; n = 4), and tonsils ( n = 4). (b) hGMDPs, hMDPs, and hCDPs were purified from human BM, and 2,500 progenitors were cultured in MS5+FSG for 7 d. Flow cytometry plot... View More

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产品信息

CD34-581-18

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL for 1x10^6 cells

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

581

抗原

Human CD34.

偶联物

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

激发/发射光谱

564/692 nm 查看光谱

形式

Liquid

浓度

0.05 mg/mL

纯化类型

purified

保存液

PBS with BSA, sucrose

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536504

靶标信息

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

TCR⁺/BCR⁺ dual-expressing cells and their associated public BCR clonotype are not enriched in type 1 diabetes.

Cell

Japp AS,Meng W,Rosenfeld AM,Perry DJ,Thirawatananond P,Bacher RL,Liu C,Gardner JS,Atkinson MA,Kaestner KH,Brusko TM,Naji A,Luning Prak ET,Betts MR

CD34-581-18 was used in Flow Cytometry to suggest that novel hybrid lymphocyte expressing both a B and T cell receptor cells and their alleged public clonotype are not enriched in T1D.

Thu Feb 04 00:00:00 UTC 2021

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

CD34-581-18 was used in Immunohistochemistry to determine whether murine adipose tissue can be used as an accurate comparative model of human disease, by comparing its architecture again human abdominal and gluteofemoral adipose tissue.

Mon Oct 01 00:00:00 UTC 2018

Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells.

Oncology letters

Chen J,Wei H,Cheng J,Xie B,Wang B,Yi J,Tian B,Liu Z,Wang F,Zhang Z

CD34-581-18 was used in Flow cytometry/Cell sorting to select and characterized a multidrug-resistant HL-60 human acute promyelocytic leukemia cell line, HL-60/RS, by exposure to stepwise incremental doses of doxorubicin.

Mon Jan 01 00:00:00 UTC 2018

Circulating precursors of human CD1c+ and CD141+ dendritic cells.

The Journal of experimental medicine

Breton G,Lee J,Zhou YJ,Schreiber JJ,Keler T,Puhr S,Anandasabapathy N,Schlesinger S,Caskey M,Liu K,Nussenzweig MC

Published figure using CD34 monoclonal antibody (Product # CD34-581-18) in Flow Cytometry

Mon Mar 09 00:00:00 UTC 2015

Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow.

The Journal of experimental medicine

Lee J,Breton G,Oliveira TY,Zhou YJ,Aljoufi A,Puhr S,Cameron MJ,Sékaly RP,Nussenzweig MC,Liu K

Published figure using CD34 monoclonal antibody (Product # CD34-581-18) in Flow Cytometry

Mon Mar 09 00:00:00 UTC 2015

Programmed cell death protein 1 and programmed death-ligand 1 are expressed on the surface of some small-cell lung cancer lines.

American journal of cancer research

Yamane H,Isozaki H,Takeyama M,Ochi N,Kudo K,Honda Y,Yamagishi T,Kubo T,Kiura K,Takigawa N

CD3458118 was used in flow cytometry to identify the presence of PD-1 and PD-L1 proteins in small-cell lung cancer cell lines.

Tue Jun 23 00:00:00 UTC 2015

Impaired immunomodulatory ability of bone marrow mesenchymal stem cells on CD4(+) T cells in aplastic anemia.

Results in immunology

Li J,Lu S,Yang S,Xing W,Feng J,Li W,Zhao Q,Wu H,Ge M,Ma F,Zhao H,Liu B,Zhang L,Zheng Y,Han ZC

Published figure using CD34 monoclonal antibody (Product # CD34-581-18) in Flow Cytometry

Fri Dec 27 00:00:00 UTC 2013

Assessment of human multi-potent hematopoietic stem/progenitor cell potential using a single in vitro screening system.

PloS one

Calvo J,BenYoucef A,Baijer J,Rouyez MC,Pflumio F

Published figure using CD34 monoclonal antibody (Product # CD34-581-18) in Flow Cytometry

Wed May 22 00:00:00 UTC 2013

Antigen CD34+ marrow cells engraft lethally irradiated baboons.

The Journal of clinical investigation

Berenson RJ,Andrews RG,Bensinger WI,Kalamasz D,Knitter G,Buckner CD,Bernstein ID

CD34-581-18 was used in flow cytometry to propose that stem cells responsible for hematopoietic reconstitution are CD34+

Tue Mar 01 00:00:00 UTC 1988

生物信息学

蛋白别名: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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基因别名: CD34

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UniProt ID: (Human) P28906

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Entrez Gene ID: (Human) 947

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功能

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

参与通路

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis

CD34 Monoclonal Antibody (581), PE-Cyanine5.5

CD34 Antibody (CD34-581-18) in Flow

产品信息

CD34-581-18

免疫组化 (IHC)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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