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CD38 Antibody (50-0380-80) in Flow
Staining of rat splenocytes with Anti-Rat CD3 PE (Product # 12-0030-82) and 0.125 µg of Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or 0.125 µg of Anti-Rat CD38 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
50-0380-80
产品规格
种属反应
Rat
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
14.27
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574158
产品详细信息
Description: The monoclonal antibody 14.27 reacts with rat CD38, also known as ADP-ribosyl cyclase. CD38 is a type II transmembrane glycoprotein that is an ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic adenosine diphosphate ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP). cADPR and NAADP are secondary messengers involved in calcium mobilization. CD38 can also act as a receptor for CD31 or agonist monoclonal antibodies. CD38 is expressed by leukocytes, early hematopoietic precursor cells, epithelial cells, astrocytes, some muscle cells, kidney cells, and thyroid cells. CD38 plays a role in the production of pro-inflammatory cytokines, proliferation, cell activation, cell migration, cell adhesion, and protection from apoptosis.
Applications Reported: This 14.27 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 14.27 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen p45; CD38H; Cyclic ADP-ribose hydrolase 1; NAD+ nucleosidase
基因别名: ADPRC 1; Cd38
UniProt ID: (Rat) Q64244
Entrez Gene ID: (Rat) 25668
response to hypoxia
positive regulation of cytosolic calcium ion concentration
female pregnancy
metabolic process
response to hormone
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of insulin secretion
response to estradiol
response to retinoic acid
response to progesterone
response to hydroperoxide
response to cytokine
response to drug
negative regulation of apoptotic process
negative regulation of bone resorption
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of vasoconstriction
B cell receptor signaling pathway
long term synaptic depression
response to interleukin-1
