Invitrogen

HA Tag Monoclonal Antibody (2-2.2.14), DyLight™ 488

HA Tag Antibody in Immunocytochemistry (ICC/IF)

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HA Tag Antibody (26183-D488) in ICC/IF

Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Monoclonal Antibody (2-2.2.14), DyLight 488 (Product # 26183-D488) at 1:200 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with ... View More

HA Tag Antibody in Immunohistochemistry (IHC)

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产品信息

26183-D488

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:25-1:200

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Tag

已发表种属

Tag

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2-2.2.14

抗原

HA peptide YPYDVPDYA derivitized to ovalbumin.

偶联物

DyLight™ 488 DyLight™ 488 DyLight™ 488

激发/发射光谱

492/519 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2533051

产品详细信息

26183-D488 has been successfully used for immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.

HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.

靶标信息

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Conserved nuclear hormone receptors controlling a novel plastic trait target fast-evolving genes expressed in a single cell.

PLoS genetics

Sieriebriennikov B,Sun S,Lightfoot JW,Witte H,Moreno E,Rödelsperger C,Sommer RJ

26183-D488 was used in Immunohistochemistry-immunofluorescence to investigate the gene regulatory network controlling predatory vs. non-predatory dimorphism in the nematode Pristionchus pacificus.

Wed Apr 01 00:00:00 UTC 2020

Conserved nuclear hormone receptors controlling a novel plastic trait target fast-evolving genes expressed in a single cell.

PLoS genetics

Sieriebriennikov B,Sun S,Lightfoot JW,Witte H,Moreno E,Rödelsperger C,Sommer RJ

26183-D488 was used in Immunohistochemistry-immunofluorescence to investigate the gene regulatory network controlling predatory vs. non-predatory dimorphism in the nematode Pristionchus pacificus.

Wed Apr 01 00:00:00 UTC 2020

mRNA as a Novel Treatment Strategy for Hereditary Spastic Paraplegia Type 5.

Molecular therapy. Methods & clinical development

Hauser S,Poenisch M,Schelling Y,Höflinger P,Schuster S,Teegler A,Betten R,Gustafsson JÅ,Hübener-Schmid J,Schlake T,Chevessier-Tünnesen F,Horscroft N,Björkhem I,Schöls L

Published figure using HA Tag monoclonal antibody (Product # 26183-D488) in Immunocytochemistry

Fri Dec 13 00:00:00 UTC 2019

Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kirmiz M,Vierra NC,Palacio S,Trimmer JS

26183-D488 was used in Immunohistochemistry-immunofluorescence to suggests that Kv2.1 expression in the PM can affect ER-PM junctions via its phosphorylation-dependent association to ER-localized VAPA and VAPB.

Wed Aug 29 00:00:00 UTC 2018