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IL-23 p19 Antibody (50-7823-42) in Flow
Intracellular staining of unstimulated (left) or LPS-stimulated and monensin-treated (right), in vitro-cultured human dendritic cells with Anti-Human IL-12/23 p40 PE (Product # 12-7235-42) and Anti-Human IL-23 p19 eFluor® 660. Cultures were fixed and permeabilized with the Fixation and Permeabilization Kit (Product # 88-8823-88). Total viable cells as determined by staining with the Fixable Viability Dye eFluor® 450 (Product # 65-0863-14) were used for analysis. Data from two donors are shown to illustrate variability in expression.
产品信息
50-7823-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Rhesus monkey
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
23dcdp
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10597593
产品详细信息
Description: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which promotes maintenance and proliferation of T helper 17 (Th17) cells.
Dendritic cells and macrophages produce high levels of p40 and low levels of IL-23 in response to TLR2, TLR4, and TLR8 ligands and agonists of the beta-glucan receptor, Dectin-1. This is due to the presence of monomer and dimer forms of p40, while only a small portion associates with p19 to form IL-23. Our studies suggest donor variability, expression kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.
Applications Reported: This 23dcdp antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 23dcdp antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of cultured human dendritic cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; p40; RP23-388G23.1
基因别名: IL-23; IL-23A; IL23A; IL23P19; P19; SGRF; UNQ2498/PRO5798
UniProt ID: (Human) Q9NPF7
Entrez Gene ID: (Human) 51561
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
positive regulation of T-helper 1 type immune response
inflammatory response
positive regulation of activation of JAK2 kinase activity
negative regulation of interleukin-10 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
positive regulation of tissue remodeling
T cell proliferation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of NF-kappaB import into nucleus
regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat4 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of memory T cell differentiation
innate immune response
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
tissue remodeling
positive regulation of inflammatory response
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
positive regulation of neutrophil chemotaxis
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 17 cell lineage commitment
