Invitrogen

IRAK1 Polyclonal Antibody

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IRAK1 Antibody (PA5-19855) in WB

Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-19855) and an 80 kDa band corresponding to IRAK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), LNCaP (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), K-562 (Lane 5), U-2 OS (Lane 6) and T-47D (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB2100... View More

IRAK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

IRAK1 Antibody — Figure 5 Analysis of E2 signal transduction. ( a ) Western blotting of A549, MCF-10A, and 293T cell lysates showing IL-1R expression; cytoplasmic and membrane components of the cell lysates were separated. A549 cells were included as a positive control for IL-1R1 expression, and 293T cells were used as a negative control. ( b ) Western blotting of MCF-10A cells showing phospho-IRAK1 (left) and IRAK1 (right) following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( c ) Western blotting of MCF-10A cells probed to examine phospho-p38(Thr180/Tyr182) and p38 after adding 100 ng/ml GST-IL-1beta for 0-60 min. ( d ) Western blotting of MCF-10A cells probed to examine phospho-p38 (Thr180/Tyr182) and p38 after adding 1 ng/ml GST-IL-1beta for 0-60 min. ( e ) Western blotting of MCF-10A cells showing phospho-JNK(Thr183/Tyr185) and JNK following treatment with 100 ng/ml GST-IL-1beta for 0-60 min. ( f ) Western blotting of MCF-10A cells showing phospho-IkB (Ser32) and IkB following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( g ) MMP-3 activity assay of MCF-10A cells following treatment with 100 ng/ml GST-IL-1beta with or without 1.6 muM NNGH, which was used as an MMP-3 inhibitor. Four independent experiments were performed. Bars represent +/-SD. ( h ) Representative confocal images of MCF-10A cells in a 3D culture treated with 100 ng/ml GST-IL-1beta (second row) or E2 (32 nM, third row) or control (first row) for 7 days to examine the basement membrane via immunofluorescenc {TM}

产品信息

PA5-19855

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫细胞化学 (ICC/IF)

10 µg/mL

免疫沉淀 (IP)

2-4 µg

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A peptide corresponding to amino acids near the carboxy terminus of human IRAK.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11152551

产品详细信息

A suggested positive control is Hela cell lysate.

PA5-19855 can be used with blocking peptide PEP-0001.

靶标信息

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis.

Cell death & disease

Wang Y,Pei S,Liu Z,Ding Y,Qian T,Wen H,Hsu SW,Zhou Z,Zhang J,Wang H

PA5-19855 was used in Western Blotting and Immunoprecipitaiton to suggest a potential target for the novel therapeutic approaches of multiple sclerosis (MS)/EAE and NLRP3-related inflammatory diseases.

Fri Feb 10 00:00:00 UTC 2023

IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis.

Cell death & disease

Wang Y,Pei S,Liu Z,Ding Y,Qian T,Wen H,Hsu SW,Zhou Z,Zhang J,Wang H

Fri Feb 10 00:00:00 UTC 2023

IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis.

Cell death & disease

Wang Y,Pei S,Liu Z,Ding Y,Qian T,Wen H,Hsu SW,Zhou Z,Zhang J,Wang H

Fri Feb 10 00:00:00 UTC 2023

Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal miR-146a-5p which targets IRAK1.

Theranostics

Li X,Liao J,Su X,Li W,Bi Z,Wang J,Su Q,Huang H,Wei Y,Gao Y,Li J,Liu L,Wang C

PA5-19855 was used in Western Blotting to study the protective effects of human urine-derived stem cells against a rat model of renal ischemia/reperfusion injury.

Tue Jun 01 00:00:00 UTC 2021

Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal miR-146a-5p which targets IRAK1.

Theranostics

Li X,Liao J,Su X,Li W,Bi Z,Wang J,Su Q,Huang H,Wei Y,Gao Y,Li J,Liu L,Wang C

PA5-19855 was used in Western Blotting to study the protective effects of human urine-derived stem cells against a rat model of renal ischemia/reperfusion injury.

Tue Jun 01 00:00:00 UTC 2021

Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Scientific reports

Deng Y,Miki Y,Nakanishi A

Published figure using IRAK1 polyclonal antibody (Product # PA5-19855) in Western Blot

Tue Jan 28 00:00:00 UTC 2020

生物信息学

蛋白别名: Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase

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基因别名: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk; RGD1563841

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UniProt ID: (Human) P51617, (Mouse) B1AUW4

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Entrez Gene ID: (Human) 3654, (Mouse) 16179, (Rat) 363520

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