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IRAK1 Monoclonal Antibody (3A9)

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抗体检测数据 (7)

IRAK1 Antibody in Immunocytochemistry (ICC/IF)

IRAK1 Antibody in Immunocytochemistry (ICC/IF)

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IRAK1 Antibody (H00003654-M02) in ICC/IF

Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration is 10 µg/mL.

IRAK1 Antibody in Immunocytochemistry (ICC/IF)

IRAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Western Blot (WB)

IRAK1 Antibody in Immunoprecipitation (IP)

IRAK1 Antibody in ELISA (ELISA)

Product Image Gallery

IRAK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration is 10 µg/mL.

IRAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. Antibody concentration is 3 µg/mL.

IRAK1 Antibody in Western Blot (WB) — Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi (Cat # H00003654-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9 (Cat # H00003654-M02). GAPDH ( 36.1 kDa ) used as specificity and loading control.

IRAK1 Antibody in Western Blot (WB) — Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.

Lane 1: IRAK1 transfected lysate(68 KDa).
Lane 2: Non-transfected lysate.

IRAK1 Antibody in Western Blot (WB) — IRAK1 monoclonal antibody (M02), clone 3A9 Western Blot analysis of IRAK1 expression in A-431 (Cat # L015V1).

IRAK1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.

IRAK1 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged IRAK1 is approximately 0.03 ng/mL as a capture antibody.

产品信息

H00003654-M02

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

-

免疫组化（石蜡） (IHC (P))

3 µg/mL

-

免疫细胞化学 (ICC/IF)

10 µg/mL

-

酶联免疫吸附实验 (ELISA)

0.03 ng/mL

-

免疫沉淀 (IP)

1-5 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3A9

抗原

IRAK1 (AAH54000, 530 a.a. approximately 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

See Label

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Sequence of this protein is as follows: SSTGRAHSGA APWQPLAAPS GASAQAAEQL QRGPNQPVES DESLGGLSAA LRSWHLTPSC PLDPAPLREA GCPQGDTAGE SSWGSGPGSR PTAVEGLALG SSASSSSEPP QIIINPARQK MVQKLALYED GALDSLQLLS SSSLPGLGLE QDRQGPEESD EFQS

靶标信息

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog

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基因别名: IRAK; IRAK1; pelle

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UniProt ID: (Human) P51617

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Entrez Gene ID: (Human) 3654

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protein kinase activity protein serine/threonine kinase activity NF-kappaB-inducing kinase activity protein binding ATP binding kinase activity heat shock protein binding protein homodimerization activity protein heterodimerization activity

activation of MAPK activity regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway protein phosphorylation signal transduction transmembrane receptor protein serine/threonine kinase signaling pathway activation of NF-kappaB-inducing kinase activity JNK cascade aging lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of type I interferon production response to lipopolysaccharide toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway toll-like receptor 9 signaling pathway cellular response to heat negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity innate immune response positive regulation of transcription, DNA-templated protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of NF-kappaB transcription factor activity protein oligomerization nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to hypoxia