Invitrogen

Nur77 Monoclonal Antibody (12.14), eBioscience™

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Nur77 Antibody (14-5965-82) in WB

Immunoblotting of PMA and ionomycin-stimulated (lane 1) and unstimulated mouse thymocytes (lane 2) with 5 µg/mL of Anti-Mouse Nur77 Purified. The band was visualized using Rat Anti-Mouse IgG HRP.

Nur77 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

14-5965-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

12.14

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1257215

产品详细信息

Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.

Applications Reported: This 12.14 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 12.14 antibody has been tested by western blot on lysates prepared from mouse thymocytes stimulated with PMA and ionomycin for two hours. This antibody can be used at 5 µg/mL. It is recommended that this antibody by titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.

Nature communications

Peng SZ,Chen XH,Chen SJ,Zhang J,Wang CY,Liu WR,Zhang D,Su Y,Zhang XK

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Immunocytochemistry

Wed Oct 13 00:00:00 UTC 2021

T cell self-reactivity during thymic development dictates the timing of positive selection.

eLife

Lutes LK,Steier Z,McIntyre LL,Pandey S,Kaminski J,Hoover AR,Ariotti S,Streets A,Yosef N,Robey EA

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Thu Apr 22 00:00:00 UTC 2021

Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.

Nucleic acids research

Patta I,Madhok A,Khare S,Gottimukkala KP,Verma A,Giri S,Dandewad V,Seshadri V,Lal G,Misra-Sen J,Galande S

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Fri Jun 19 00:00:00 UTC 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Tue Nov 17 00:00:00 UTC 2020

CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

The Journal of clinical investigation

Halkias J,Rackaityte E,Hillman SL,Aran D,Mendoza VF,Marshall LR,MacKenzie TC,Burt TD

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Thu May 30 00:00:00 UTC 2019

IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate.

eLife

Noviski M,Mueller JL,Satterthwaite A,Garrett-Sinha LA,Brombacher F,Zikherman J

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Fri Mar 09 00:00:00 UTC 2018

Capacity of tTreg generation is not impaired in the atrophied thymus.

PLoS biology

Oh J,Wang W,Thomas R,Su DM

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Wed Nov 01 00:00:00 UTC 2017

CD6 modulates thymocyte selection and peripheral T cell homeostasis.

The Journal of experimental medicine

Orta-Mascaró M,Consuegra-Fernández M,Carreras E,Roncagalli R,Carreras-Sureda A,Alvarez P,Girard L,Simões I,Martínez-Florensa M,Aranda F,Merino R,Martínez VG,Vicente R,Merino J,Sarukhan A,Malissen M,Malissen B,Lozano F

14-5965 was used in Flow cytometry/Cell sorting to analyse the in vivo consequences of CD6 deficiency.

Mon Jul 25 00:00:00 UTC 2016

Higher Sensitivity of Foxp3+ Treg Compared to Foxp3- Conventional T Cells to TCR-Independent Signals for CD69 Induction.

PloS one

Bremser A,Brack M,Izcue A

14-5965 was used in Flow cytometry/Cell sorting to investigate the effects of TCR signals on T cells through studying the expression of T cell activation marker CD69.

Tue May 24 00:00:00 UTC 2016

Functionally responsive self-reactive B cells of low affinity express reduced levels of surface IgM.

European journal of immunology

Kirchenbaum GA,St Clair JB,Detanico T,Aviszus K,Wysocki LJ

14-5965 was used in Flow cytometry/Cell sorting to suggest that low affinity, self-reactive B cells downmodulate sIgM to preserve their immunocompetence.

Tue Apr 01 00:00:00 UTC 2014

Diacylglycerol kinase ζ limits the generation of natural regulatory T cells.

Science signaling

Schmidt AM,Zou T,Joshi RP,Leichner TM,Pimentel MA,Sommers CL,Kambayashi T

14-5965 was used in Flow cytometry/Cell sorting to identify critical signal events in nT(reg) cell development, showing that diacylglycerol kinase ζ limits the generation of nT(reg) cells.

Tue Nov 26 00:00:00 UTC 2013

NR4A nuclear receptors support memory enhancement by histone deacetylase inhibitors.

The Journal of clinical investigation

Hawk JD,Bookout AL,Poplawski SG,Bridi M,Rao AJ,Sulewski ME,Kroener BT,Manglesdorf DJ,Abel T

14-5965 was used in Western Blotting to demonstrate that the Nr4a gene family contributes to memory formation and is a promising target for improving cognitive function.

Mon Oct 01 00:00:00 UTC 2012

Reduced TCR signaling potential impairs negative selection but does not result in autoimmune disease.

The Journal of experimental medicine

Hwang S,Song KD,Lesourne R,Lee J,Pinkhasov J,Li L,El-Khoury D,Love PE

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Flow Cytometry

Mon Sep 24 00:00:00 UTC 2012

The orphan nuclear receptor NUR77 regulates hormone-induced StAR transcription in Leydig cells through cooperation with Ca2+/calmodulin-dependent protein kinase I.

Molecular endocrinology (Baltimore, Md.)

Martin LJ,Boucher N,Brousseau C,Tremblay JJ

Published figure using Nur77 monoclonal antibody (Product # 14-5965-82) in Western Blot

Mon Sep 01 00:00:00 UTC 2008

生物信息学

蛋白别名: hormone receptor; MGC9485; Nuclear hormone receptor NUR/77; Nuclear protein N10; Nuclear receptor subfamily 4 group A member 1; Orphan nuclear receptor HMR; Orphan receptor tr3

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基因别名: Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; Nr4a1; Nur77; TIS1; TR3

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UniProt ID: (Mouse) P12813

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Entrez Gene ID: (Mouse) 15370

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功能