Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 2
S100A8 Antibody (50-9745-42) in Flow
Normal human peripheral blood cells were incubated with Human Fc Receptor Binding Inhibitor Purified (Product # 14-9161-73) for 20 minutes, then stained with Anti-Human CD11b PE (Product # 12-0118-42) followed by fixation and permeabilization with the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. The cells were then stained with Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or Anti-Human S100A8 eFluor® 660 (right). Cells in the monocyte gate were used for analysis.
产品信息
50-9745-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
CF-145
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574354
产品详细信息
Description: The monoclonal antibody CF-145 recognizes human S100A8. S100A8, also known as migration inhibitory factor-related protein 8 (MRP-8) or Calgranulin-A, is a calcium binding protein and member of the S100 family of proteins. S100A8 is expressed by neutrophils, monocytes, macrophages, granulocytes, and epithelial cells during dermatoses, such as psoriasis. S100A8 forms a heterodimer with S100A9, which together is known as calprotectin. S100A8 has antimicrobial and pro-apoptotic properties and is a pro-inflammatory mediator in acute and chronic inflammation. Levels of S100A8 are elevated in patients with various inflammatory disorders such as cystic fibrosis, rheumatoid arthritis, and chronic bronchitis. Upregulation of S100A8 is also observed in many tumors, including skin, breast, colon, pancreatic, and ovarian cancers.
Applications Reported: This CF-145 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.
Applications Tested: This CF-145 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: calgranulin A; Calgranulin-A; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; Protein S100-A8; S100 A8; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); Urinary stone protein band A
基因别名: 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; S100A8
UniProt ID: (Human) P05109
Entrez Gene ID: (Human) 6279
cytokine production
leukocyte migration involved in inflammatory response
acute inflammatory response
chronic inflammatory response
positive regulation of peptide secretion
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
response to zinc ion
astrocyte development
peptidyl-cysteine S-nitrosylation
positive regulation of cell growth
neutrophil chemotaxis
sequestering of zinc ion
response to lipopolysaccharide
chemokine production
wound healing
defense response to bacterium
innate immune response
response to ethanol
positive regulation of inflammatory response
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
