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phospho-Tyrosine Antibody (14-5001-82) in WB
Immunoblotting of reduced lysates from mouse splenocytes left untreated (lane 1) or treated with hydrogen peroxide-activated sodium orthovanadate (lane 2), and probed with 1 µg/mL of Anti-Human/Mouse Phosphotyrosine Purified. Bands were visualized using Rat Anti-Mouse IgG HRP.
产品信息
14-5001-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical,
Mouse
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
pY20
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572884
产品详细信息
Description: The pY20 monoclonal antibody recognizes phosphorylated tyrosine residues (p-Tyr) on proteins. Numerous intracellular signaling cascades are propagated via phosphorylation of specific tyrosine on signaling proteins. The detection of p-Tyr residues is valuable for the characterization and purification of phosphorylated proteins and the biochemical pathways that they are involved in.
Applications Reported: This pY20 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting.
Applications Tested: This pY20 antibody has been tested by immunoblotting of mouse spleen cells left untreated or treated with the phosphatase inhibitor sodium orthovanadate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Phosphotyrosine; pTyr; pY
