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B7-H6 Antibody (46-6526-42) in Flow
HeLa cells were stained with Mouse IgG1 kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4714-82) (blue) or B7-H6 Monoclonal Antibody, PerCP-eFluor 710 (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), were used for analysis.
产品信息
46-6526-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
JAM1EW
抗原
B7-H6 recombinant protein
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2784692
产品详细信息
Description: This JAM1EW monoclonal antibody recognizes human B7-H6 (NCR3LG1) protein on the surface of cells. It recognizes protein on live but not on paraformaldehyde-fixed cells. The JAM1EW clone is specific to B7-H6. When tested in flow cytometry against cells transfected with either B7-H4, B7-H5, B7-H6, or B7-H7, clone JAM1EW only stains positively on B7-H6 transfected cells.
Applications Tested: This JAM1EW antibody has been pre-diluted and tested by flow cytometric analysis of the HL-60 cell line. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
靶标信息
B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B7 homolog 6; B7-H6; DKFZp686I21167; Natural cytotoxicity triggering receptor 3 ligand 1; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
基因别名: B7-H6; B7H6; DKFZp686O24166; NCR3LG1
UniProt ID: (Human) Q68D85
Entrez Gene ID: (Human) 374383
