Invitrogen

CD4 Monoclonal Antibody (N1UG0), Biotin, eBioscience™

CD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD4 Antibody (13-2444-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 10 µg/mL of Anti-Human CD4 Biotin, followed by Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.

CD4 Antibody in Immunohistochemistry (IHC)

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

CD4 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD8 PE and CD4 Biotin followed by staining with APC conjugated streptavidin. As expected based on known relative expression patterns, CD4 clone N1UG0 stains a subset of lymphocytes (pink) and monocytes (orange), but not granulocytes (blue). {RE}

产品信息

13-2444-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

N1UG0

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572780

产品详细信息

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections.

Applications Reported: This N1UG0 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This N1UG0 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. The N1UG0 antibody has been tested by flow cytometric analysis of human PBMC. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。