Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), FITC, eBioscience™

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IL-6 Antibody (11-7069-82) in Flow

Intracellular staining of normal human peripheral blood cells unstimulated (left) or stimulated with LPS and monensin (right) with Anti-Human CD14 PerCP-Cy5-5 (Product # 45-0149-42) and Anti-Human IL-6 FITC. Cells in the monocyte gate were used for analysis.

IL-6 Antibody in Immunocytochemistry (ICC/IF)

IL-6 Antibody in T-Cell Activation (TCA)

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产品信息

11-7069-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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酶联免疫吸附实验 (ELISA)

查看2篇已发表文章 2篇已发表文章

T细胞活化 (TCA)

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MQ2-13A5

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465397

产品详细信息

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported:MQ2-13A5 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by intracellular flow cytometric analysis of stimulated normal human perippheral blood cells. This can be used at test size: 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

BCG revaccination in adults enhances pro-inflammatory markers of trained immunity along with anti-inflammatory pathways.

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Ahmed A,Tripathi H,van Meijgaarden KE,Kumar NC,Adiga V,Rakshit S,Parthiban C,Eveline J S,D'Souza G,Dias M,Ottenhoff THM,Netea MG,Joosten SA,Vyakarnam A

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in Flow Cytometry

Fri Oct 20 00:00:00 UTC 2023

Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer.

Cancer science

Zhang GQ,Jiao Q,Shen CT,Song HJ,Zhang HZ,Qiu ZL,Luo QY

11-7069 was used in Flow cytometry/Cell sorting to investigate the expression of IL-6 and PD-L1 in thyroid cancer, and whether IL-6 regulates the PD-L1 expression.

Mon Mar 01 00:00:00 UTC 2021

Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer.

Cancer science

Zhang GQ,Jiao Q,Shen CT,Song HJ,Zhang HZ,Qiu ZL,Luo QY

11-7069 was used in Flow cytometry/Cell sorting to investigate the expression of IL-6 and PD-L1 in thyroid cancer, and whether IL-6 regulates the PD-L1 expression.

Mon Mar 01 00:00:00 UTC 2021

PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages.

Scientific reports

Obst J,Hall-Roberts HL,Smith TB,Kreuzer M,Magno L,Di Daniel E,Davis JB,Mead E

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in ELISA

Wed Oct 06 00:00:00 UTC 2021

3D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization.

Pharmaceutics

Jiang Q,Bai G,Liu X,Chen Y,Xu G,Yang C,Zhang Z

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in Flow Cytometry

Tue Nov 16 00:00:00 UTC 2021

Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells.

Stem cells (Dayton, Ohio)

Jiang C,Zhang Q,Shanti RM,Shi S,Chang TH,Carrasco L,Alawi F,Le AD

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in Immunofluorescence

Fri Sep 01 00:00:00 UTC 2017

Decitabine, a DNA-demethylating agent, promotes differentiation via NOTCH1 signaling and alters immune-related pathways in muscle-invasive bladder cancer.

Cell death & disease

Ramakrishnan S,Hu Q,Krishnan N,Wang D,Smit E,Granger V,Rak M,Attwood K,Johnson C,Morrison C,Pili R,Chatta G,Guru K,Gueron G,McNally L,Wang J,Woloszynska-Read A

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in T-Cell Activation

Thu Dec 14 00:00:00 UTC 2017

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,Güzel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,Özen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in ELISA

Tue Sep 06 00:00:00 UTC 2016

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in ELISA

Tue Sep 06 00:00:00 UTC 2016

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using IL-6 monoclonal antibody (Product # 11-7069-82) in Flow Cytometry

Mon Sep 08 00:00:00 UTC 2014

Early life ozone exposure results in dysregulated innate immune function and altered microRNA expression in airway epithelium.

PloS one

Clay CC,Maniar-Hew K,Gerriets JE,Wang TT,Postlethwait EM,Evans MJ,Fontaine JH,Miller LA

11-7069-82 was used in Immunohistochemistry-immunofluorescence to examine the mechanisms behind the enhancement of susceptibility to respiratory morbidity and exposure to ozone in early life.

Mon Feb 09 00:00:00 UTC 2015

生物信息学

蛋白别名: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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基因别名: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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功能

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

参与通路