stylized image representing Nucleic Acid Purification

MagJET DNA and RNA purification kits

Nucleic acid isolation is one of the most fundamental steps in molecular biology applications and is a prerequisite for many experiments. MagJET nucleic acid purification kits utilize magnetic bead–based technology, allowing for highly efficient nucleic acid isolation from a variety of samples at any throughput. MagJET kits provide high-purity DNA and RNA ready to use in routine and demanding downstream applications.

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Features & benefits of MagJET magnetic bead-based purification

MagJET magnetic bead-based technology utilizes proprietary high capacity paramagnetic particles optimized to isolate nucleic acids with superior purity and yields compared to other kits on the market. Due to large total surface area, MagJET magnetic beads exhibit high binding capacity, resulting in superior nucleic acid yields and recovery rates typically exceeding 80%. Uniform bead size and shape ensure robust performance and consistent results with less sample-to-sample variation. MagJET magnetic beads are highly stable in solution and respond rapidly to an applied magnetic field making MagJET nucleic acid purification kits an ideal choice for high-throughput automation.

A MagJET bead cut in half showing the magnetic core

Features:

  • High binding capacity
  • Fast magnetic response
  • Uniform size and shape
  • Ideal for automation
  • High recovery (>80%)
  • Proprietary technology

MagJET nucleic acid isolation technology is suitable both for high-throughput automated and manual sample processing.

Diagram of high-throughput automated and manual sample processing using magnetic beads.

(A) The automation protocols are optimized for Thermo Scientific Kingfisher instruments where magnetic beads are transferred from well to well during the purification procedure.

(B) In the manual protocol a magnetic field applied by an external source is used to capture the beads against the wall of the tube. The supernatant is removed by aspiration.

Manual and automated workflows were used to purify RNA from N. tabacum leaf samples (each 50 mg) with the MagJET Plant RNA Kit. The Thermo Scientific KingFisher protocol was used for the automated workflow. Isolated RNA was analyzed by agarose gel electrophoresis (1%), M – Thermo Scientific RiboRuler High Range RNA Ladder.

Manual and automated workflows using the MagJET Plant RNA Kit analyzed by agarose gel electrophoresis.

MagJET magnetic bead-based technology utilizes proprietary high capacity paramagnetic particles optimized to isolate nucleic acids with superior purity and yields compared to other kits on the market. Due to large total surface area, MagJET magnetic beads exhibit high binding capacity, resulting in superior nucleic acid yields and recovery rates typically exceeding 80%. Uniform bead size and shape ensure robust performance and consistent results with less sample-to-sample variation. MagJET magnetic beads are highly stable in solution and respond rapidly to an applied magnetic field making MagJET nucleic acid purification kits an ideal choice for high-throughput automation.

A MagJET bead cut in half showing the magnetic core

Features:

  • High binding capacity
  • Fast magnetic response
  • Uniform size and shape
  • Ideal for automation
  • High recovery (>80%)
  • Proprietary technology

MagJET nucleic acid isolation technology is suitable both for high-throughput automated and manual sample processing.

Diagram of high-throughput automated and manual sample processing using magnetic beads.

(A) The automation protocols are optimized for Thermo Scientific Kingfisher instruments where magnetic beads are transferred from well to well during the purification procedure.

(B) In the manual protocol a magnetic field applied by an external source is used to capture the beads against the wall of the tube. The supernatant is removed by aspiration.

Manual and automated workflows were used to purify RNA from N. tabacum leaf samples (each 50 mg) with the MagJET Plant RNA Kit. The Thermo Scientific KingFisher protocol was used for the automated workflow. Isolated RNA was analyzed by agarose gel electrophoresis (1%), M – Thermo Scientific RiboRuler High Range RNA Ladder.

Manual and automated workflows using the MagJET Plant RNA Kit analyzed by agarose gel electrophoresis.
MagJET Plasmid DNA Kit

MagJET Plasmid DNA Kit

The MagJET Plasmid DNA Kit is ideal for high-throughput plasmid DNA miniprep isolation from 0.5 to 5 mL of bacterial culture. Isolated plasmid DNA is compatible with sensitive downstream applications such as PCR, restriction enzyme digestion and transfection of robust cell lines.

View some tips and tricks for plasmid DNA purification using the Magjet Plasmid DNA Kit in this video.

Features:

  • High yields–up to 5 µg of plasmid DNA from 1 mL of bacterial culture
  • Pure–isolated DNA is immediately ready-to-use in downstream application such as sequencing, in vitro transcription, restriction enzyme digestion, and or transfection of robust cell lines
  • Flexible–automated and manual processing protocols available
  • Reproducible results and robust performance
  • Includes RNase A

MagJET NGS Cleanup and Size Selection Kit

MagJET NGS Cleanup and Size Selection Kit

The MagJET NGS Cleanup and Size Selection Kit is optimized for robust and efficient DNA fragment library purification and size selection from a large variety of enzymatic reaction mixtures used in NGS workflows.

Features:

  • Robust–reliable cleanup and size selection from different reaction mixtures and buffers
  • Flexible–user-defined size-selection of exact DNA fragment range
  • NGS-grade purity–complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting the NGS workflow
  • High-throughput compatible–easy to automate on Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling

MagJET Separation Rack

A magnetic separation rack intended for low-throughput magnetic bead manipulation procedures such as magnetic bead-based nucleic acid isolation.


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Dynabeads kits and accessories

Thermo Fisher Scientific offers magnetic bead-based nucleic acid purification kits utilizing Dynabeads technology. These kits utilize Dynabeads, which are uniform, superparamagnetic beads composed of a polystyrene core embedded with magnetic nanoparticles and coated with specific ligands or antibodies. Using Dynabeads kits for magnetic bead-based nucleic acid isolation method offers a powerful and efficient approach for extracting nucleic acids from diverse sample types, helping provide high purity and yield necessary for a wide range of biological and clinical applications.

View Dynabeads magnetic bead separation technology



MagMAX kits and accessories

Thermo Fisher Scientific offers magnetic bead-based nucleic acid purification kits utilizing MagMAX technology. MagMAX kits are specialized products designed for the efficient isolation and purification of nucleic acids using magnetic bead technology. These kits are widely used in research, clinical diagnostics, and other molecular biology applications due to their robustness, scalability, and ability to deliver high-quality nucleic acids. Their versatility, scalability, and compatibility with automated workflows make them exceptional for a wide range of applications in research and clinical diagnostics.

View MagMAX kits for automated nucleic acid extraction kits


Resources

View additional resources related to nucleic acid purification.

仅供科研使用,不可用于诊断目的。