Reveal what matters with effortless selectivity
FAIMS Pro interface

FAIMS Pro interface delivers maximum usability and selectivity for proteomics workflows.

Achieve the ultimate in LC-MS performance and ion mobility spectrometry when you utilize the tool-free, one-way assembly of Thermo Scientific FAIMS Pro interface, a field asymmetric ion mobility spectrometry (FAIMS) technique that seamlessly works with select Thermo Scientific mass spectrometers. Together they further enhance superior selectivity and enable greater proteome profiling while reducing time consuming sample preparation steps.

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Key benefits of the FAIMS Pro interface
Protect precious samplesFast, higher quality resultsLow cost and improved ease of operationFocus on science, not setup
The FAIMS Pro technology is the only interface exclusively designed to improve nano, capillary, and microflow applications, allowing you to preserve precious, size-limited samples while achieving higher data quality.The unique design of FAIMS Pro technology enhances instrument selectivity and detection limits using gas phase fractionation. This results in reduced matrix interference and higher quality data, faster.Increased selectivity allows you to improve performance and save time by avoiding or reducing offline sample fractionation preparation steps. No consumable parts means lower long-term cost and maintenance.Designed for inexperienced users to easily install and use. Leverage menu-driven software to design methods using pre-configured parameter recommendations and quickly achieve results.

How does the FAIMS Pro interface work?

The FAIMS Pro interface is easy to set up and deploy to enhance experimental performance and is perfectly suited for nano to micro flow applications (up to 25µL/min). Better selectivity means increased productivity for every proteomic user.

Conventional versus differential ion mobility spectrometry (FAIMS)

Identifying and characterizing proteins and post-translational modifications by bottom-up mass spectrometry relies on the acquisition of high quality MS and MS/MS data. The FAIMS Pro interface increases analytical performance through gas phase fractionation and selective enhancement of peptidic compounds, reducing the complexity of the MS spectra, and improving analyte signal/noise ratio. The end result is greater proteome coverage.

Proteome analysis using the FAIMS Pro interface increases proteome coverage for both low and high sample loading amounts. Comparative analysis of the number of proteins and peptides confidently identified in an analysis of a tryptic digest of HeLa cell lysate under identical chromatographic conditions. While the proteome coverage measured using the standard DDA method at low and high sample loading amounts is impressive, simply incorporating the FAIMS Pro interface substantially increased protein and peptide coverage with almost 8,000 proteins confidently measured in the 140-minute method.

Incorporation of the FAIMS Pro interface into an existing DDA experimental workflow increases proteome profiling efficiency. Compared to using a standard DDA method, the FAIMS Pro interface method provided similar proteome coverage while using substantially less sample and shorter chromatographic gradients, addressing the two primary concerns for translational proteomics. Comparison of protein and peptide identifications using conventional setup (control) or FAIMS Pro interface (FAIMS) with 3 CVs (-50, -65, and -85 V). The addition of the FAIMS Pro interface enables an improvement of 18% in protein IDs and 29% in peptide IDs when compared to not using FAIMS in DDA mode, with a 140-minute nanoflow gradient on the Orbitrap Fusion Lumos MS.

Featured videos

Dr. Devin Schweppe of Harvard Medical School discusses Thermo Scientific FAIMS Pro interface for improved quantitative accuracy and peptide quantitation.

Scott Pederman discusses the Thermo Scientific FAIMS Pro interface for improved quantitative accuracy and maximum ion transmission and proteome coverage.

FAIMS Pro interface product & ordering guide

Through accessing Xcalibur software revision 3.1 or greater and Proteome Discoverer software revision 2.3 or greater, you can automate tuning and optimization, leverage data-acquisition method template recommendations and streamline data processing making setup and experimental success to results easier.

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