Our hepatic cell culture reagents help ensure the success of your metabolism studies. They have been optimized for use with our hepatocyte products by our scientific team allowing for the highest quality results.
We also offer a number of related hepatic cell culture media & reagents for customers isolating their own liver cells.
Williams Medium E
We recommend phenol red-free Williams Medium E. This is a nutrient-rich media for hepatocyte research involving LC-MS/MS analysis.
Hepatocyte Thaw Medium (HTM)
Hepatocyte Thaw Medium (HTM) is a proprietary formulation designed to enhance the recovery of viable hepatocytes while removing cryoprotectant after cell cryopreservation. HTM has proven to result in healthier hepatocytes with consistently higher viability.
CHRM® Medium (Cryopreserved Hepatocyte Recovery Medium)
Cryopreserved Hepatocyte Recovery Medium (CHRM®) is a proprietary formulation designed to enhance the recovery of viable hepatocytes while removing cryoprotectant after cell cryopreservation. When used appropriately, CHRM® has proven to result in healthier hepatocytes with consistently higher viability (Figure 1).
Figure 1: Comparison of CHRM® against DMEM in the ability to enhance hepatocyte viability post-cryopreservation. Data shown is an average of 126 studies of individual lots of human cryopreserved hepatocytes.
CHRM® is a trademark of APSciences Inc.
HepExtend™ Supplement (50X)
HepExtend™ Supplement (50X) is an enriched supplement designed to enhance the culture lifespan of cryopreserved primary hepatocytes. When cultured in HepExtend™ Supplement in conjunction with William’s E Medium and the Hepatocyte Maintenance Supplement Pack, primary human hepatocyte lots will live and be functional for 10 days or longer depending on the lot of cells and other assay conditions. HepExtend™ supplement does not contain growth factors, cytokines, fetal bovine serum, or pharmaceutical small molecules.
Hepatocyte Thawing & Plating Supplement Pack
Hepatocyte Thawing & Plating Supplement Packs contain prequalified fetal bovine serum, dexamethasone, and a cocktail solution of penicillin-streptomycin, bovine insulin, GlutaMAX™ and HEPES to supplement up to 500 mL of Williams Medium E without phenol red, or suitable alternative basal medium, for the purpose of plating fresh or cryopreserved hepatocytes.
Hepatocyte Maintenance Supplement Pack
Hepatocyte Maintenance Supplement Packs contain dexamethasone and a cocktail solution of penicillin-streptomycin, ITS+ (insulin, transferrin, selenium complex, BSA and linoleic acid), GlutaMAX™ and HEPES to supplement up to 500 mL of Williams Medium E without phenol red, or suitable alternative basal medium, for the purpose of incubating hepatocytes in suspension or plated cultures.
Collagen I, Rat Tail for Cell Culture
Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment and differentiation. In addition to our 5 mg/mL Collagen I solution, we also offer Collagen I precoated 6-, 24-, and 96-well plates for your hepatocyte experiments.
Geltrex™ Reduced Growth Factor Basement Membrane Matrix
Geltrex™ Matrix is a soluble form of reduced growth factor (RGF) basement membrane extract (BME) purified from continuous sheets of specialized extracellular matrix that form an interface between Engelbreth-Holm-Swarm (EHS) tumor cells. The Geltrex™ Matrix provides the foundation for three-dimensional (3D) culture studies.
Our video demonstration on thawing and plating cryopreserved hepatocytes