Qubit 荧光计技术资源

• Qubit 4型产品说明
• Qubit 4型快速入门指南

• 荧光定量与UV吸光度测定的比较——Qubit荧光定量 vs. 分光光度计测定
• 存在单链DNA时Qubit dsDNA检测的特异性
• 比较Quant-iT和Qubit DNA定量检测的准度和精度
• 使用Qubit荧光定量仪检测时Qubit DNA和RNA检测试剂中糖原和 GlycoBlu试剂的应用
• Qubit 荧光定量仪 vs. Quantus荧光定量仪
• 准确灵敏的蛋白质定量——用于Qubit荧光定量仪的Qubit蛋白质检测法与其他传统蛋白质检测方法的比较
• 使用单链和双链RNA均聚物的Qubit RNA检测试剂偏向性测试
• Qubit RNA IQ Assay：一种快速、简单的荧光 RNA 质量分析方法
• 移液方法和体积对核酸定量检测结果的影响

• 用于评估RNA质量的Qubit RNA IQ检测
• 快速而简便的荧光法RNA质量评估

问：我已经有一台Qubit荧光定量仪，Qubit 4型荧光定量仪有什么不同之处？
答：Qubit 4型荧光定量仪拥有更大的颜色更鲜艳的触摸屏，可以实现无缝的工作流程设置，并且可以将数据导出至USB移动存储设备或通过USB数据线直接导出至您的电脑，从而实现更高效的数据管理。此外，这款设备支持个性化，可以只显示常用的检测项目，也可添加包括用户使用MyQubit检测设计工具创建的自定义检测项目，更可使用您选定的语言进行显示，包括英语、简体中文、法语、西班牙语、意大利语、德语及日语。

问：我是否可以在Qubit 4型荧光定量仪中使用过去的标记了“用于Qubit荧光定量仪”的Quant-iT试剂盒？
答：可以，这些试剂盒可以在所有型号Qubit荧光定量仪中工作。

问：Qubit荧光定量仪可以储存多少行数据？
答：Qubit 4型和3.0型荧光定量仪可以以.csv文件的方式保存多至1000例样品的数据。
Qubit 2.0型荧光定量仪可以以.csv文件的方式保存最多200行数据。
最初的Qubit荧光定量仪则可以以.csv文件的方式保存最多20行数据。

问：我是否每次都需要使用新的标准品？
答：不用。不过我们确实建议您每次配置新的工作溶液后使用新的标准品，这样标准品中所用的工作溶液就可与您样品中使用的保持一致。

问：稀释后的标准品可以保存和重复使用多长时间？如果过段时间后出现了蒸发情况呢（明显或不明显）？
答：稀释后的标准品可以使用3个小时，同时样品都需要使用相同的工作溶液。

问：Qubit荧光定量是否可以定量质粒？
答：可以。对于含大量DNA（超过50 ng/μL）的质粒小量制备样本可以使用Qubit DNA BR检测试剂。对于“plasmid rescue”或其他仅获得少量DNA的提取方法可以使用Qubit DNA HS检测试剂。

问：超螺旋状的DNA和松弛状的DNA之间是否存在信号差异？
答：存在。对于超螺旋DNA，我们推荐进行剪切以使DNA去超螺旋化，或者使用超螺旋DNA作为2号标准品。

问：Qubit蛋白质检测试剂在去污剂存在时是否可以正常工作？
答：该款试剂可以承受非常少量的去污剂。您可以查看Qubit蛋白质检测试剂盒产品手册第6页的表2“Qubit蛋白质检测试剂可承受的污染物”来获取具体数值。

问：灯泡可以工作多久？如何更换灯泡？
答：Qubit 2.0、3.0及4型荧光定量仪中有两个光源——都是LED。它们应当可以正常工作至少5年。

问：我可以自己修理Qubit荧光定量仪吗？
答：不可以。当设备被拆解或用户尝试自行修理设备时保修会失效。

问：如果我的Qubit荧光定量仪在保修期内损坏了怎么办？
答：我们将为您更换一台Invitrogen Qubit荧光定量仪。请联系技术支持了解详情。

问：我需要购买哪种管？
答：可以使用薄壁透明的0.5 mL PCR管，例如Qubit检测管或Axygen PCR-05-C管。

• Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

• Pérez-Barrios C, Nieto-Alcolado I, et al.(2016) Comparison of methods for circulating cell-free DNA isolation using blood from cancer patients: impact on biomarker testing.  Transl Lung Cancer Res.2016 Dec;5(6):665-672.
• Kumar D, Panigrahi MK, et al.(2016) Quantification of DNA Extracted from Formalin Fixed Paraffin-Embeded Tissue Comparison of Three Techniques: Effect on PCR Efficiency.J Clin Diagn Res.2016 Sep;10(9):BC01-BC03.
• Nakayama Y, Yamaguchi H, et al.(2016) Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions.  PLoS One.2016 Mar 3;11(3):e0150528.
• Ji-Yeon Kim,, Kyunghee Park, , et al.(2016) Association between Mutation and Expression of TP53 as a Potential Prognostic Marker of Triple-Negative Breast Cancer Cancer Res Treat.2016 Oct; 48(4): 1338–1350.
• Yuxin Yin, James H. Lan, et al.(2016) Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples  PLoS One.2016; 11(10): e0165810.
• A. Sorana Morrissy, Livia Garzia, et al.(2016) Divergent clonal selection dominates medulloblastoma at recurrence.Nature.2016 Jan 21; 529(7586): 351–357.

• Spencer A, Mithraprabhu S, et al.(2015) Evaluation of circulating tumour DNA for the mutational characterisation of multiple myeloma.Blood, 126: 368.
• Kapp JR, Diss T, et al.(2015) Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial.J. Clin.Pathol 68:111-118.
• Oh JE, Ohta T, et al.(2015) Alterations in the NF2/LATS1/LATS2/YAP Pathway in Schwannomas.J Neuropathol Exp Neurol 74: 952-959. 
• Bjorn-Mortensen K, Zallet J, et al.(2015) Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing.J. Clin.Microbiol.53: 2716-2719.
• Camp JG, Badsha F, Florio M, et al.(2015) Human cerebral organoids recapitulate gene expression programs of fetal neocortex development.PNAS 112:15672-15677.
• Forero-Torres A, Varley KE, Abramson G, et al.(2015) TBCRC 019: A Phase II Trial of Nanoparticle Albumin-Bound Paclitaxel with or without the Anti-Death Receptor 5 Monoclonal Antibody Tigatuzumab in Patients with Triple-Negative Breast Cancer ClinCancer Res 21:2722-2729.
• Rowan BA, Patel V, Weigel D, et al.(2015) Rapid and Inexpensive Whole-Genome Genotyping-by-Sequencing for Crossover Localization and Fine-Scale Genetic Mapping.g3 5:385-398.
• Romero-Maraccini OC, Shisler JL, Nguyen TH, et al.(2015) Solar and Temperature Treatments Affect the Ability of Human Rotavirus Wa To Bind to Host Cells and Synthesize Viral RNA.Appl Envir Microbiol 81:4090-4097.
• Simon A, Bindschedler S, Job D, et al.(2015) Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium.  FEMS Microbiol Ecol 91:fiv116.
• Goodwin S, Gurtowski J, Ethe-Sayers S, et al.(2015) Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome.  Genome Res 25:1750-1756.
• Peng X, Wu J, Brunmeir R, et al.(2015) TELP, a sensitive and versatile library construction method for next-generation sequencing.  Nucleic Acids Res 43:e35.
• Legendre M, Lartigue A, Bertaux L, et al.(2015) In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba.PNAS   112:E5327-E5335.
• Lam V, Gomez MS, Graham SW, et al.(2015) The Highly Reduced Plastome of Mycoheterotrophic Sciaphila (Triuridaceae) Is Colinear with Its Green Relatives and Is under Strong Purifying Selection.Genome Biol Evol 7:2220-2236.
• Andrade S, Novo M, Kawauchi GY, et al.(2015) Articulating "Archiannelids": Phylogenomics and Annelid Relationships, with Emphasis on Meiofaunal Taxa.Mol Biol Evol 32:2860-2875.
• Putnam H and Gates RD (2015) Preconditioning in the reef-building coral Pocillopora damicornis and the potential for trans-generational acclimatization in coral larvae under future climate change conditions.J Exp Biol 218:2365-2372.
• Sillence KA, Roberts LA, Hollands HJ, et al.(2015) Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR.Clin Chem 61:1399-1407.
• Tosar J, Gámbaro F, Sanguinetti J, et al.(2015) Assessment of small RNA sorting into different extracellular fractions revealed by high-throughput sequencing of breast cell lines.Nucleic Acids Res 43:5601-5616.
• Bhusari S, P Andiri AR, Nagai H, et al.(2015) Genomic Profiling Reveals Unique Molecular Alterations in Hepatoblastomas and Adjacent Hepatocellular Carcinomas in B6C3F1 Mice.Toxicol Pathol 43:1114-1126.
• Li C, Corrigan S, Yang L, et al.(2015) DNA capture reveals transoceanic gene flow in endangered river sharks.PNAS  112:13302-13307.
• Rivière A, Gagnon M, Weckx S, et al.(2015) Mutual Cross-Feeding Interactions between Bifidobacterium longum subsp.  longum NCC2705 and Eubacterium rectale ATCC 33656 Explain the Bifidogenic and Butyrogenic Effects of Arabinoxylan Oligosaccharides.Appl Envir Microbiol 81:7767-7781.
• Dai K, He LA, Khan SN, et al.(2015) Rhesus Macaque B-Cell Responses to an HIV-1 Trimer Vaccine Revealed by Unbiased Longitudinal Repertoire Analysis.mBio 6:e01375-15.
• Leray M and Knowlton N. (2015) DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity.PNAS 112:2076-2081.
• González V, Andrade SCS, Bieler R, et al.(2015) A phylogenetic backbone for Bivalvia: an RNA-seq approach.Proc R Soc B 282:20142332.
• Sant DW, Margraf Rl, Stevenson DA, et al.(2015) Evaluation of somatic mutations in tibial pseudarthrosis samples in neurofibromatosis type 1.J Med Genet 52:256-261.
• Nys J, Smolders K, Laramée M, et al.(2015) Regional Specificity of GABAergic Regulation of Cross-Modal Plasticity in Mouse Visual Cortex after Unilateral Enucleation.J Neurosci 35:11174-11189.
• Stoy C, Sundaram A, Garcia MR, et al.(2015) Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy.EMBO Mol Med 7:1048-1062.
• Shi X, Hanson MR, Bentolila S, et al.(2015) Two RNA recognition motif-containing proteins are plant mitochondrial editing factors.Nucleic Acids Res 43:3814-3825.
• Higa-Nakamine S, Maeda N, Toku S, et al.(2015) Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone.J Biol Chem 290:25974-25985.
• Trost B, Moir CA, Gillespie ZE, et al.(2015) Concordance between RNA-sequencing data and DNA microarray data in transcriptome analysis of proliferative and quiescent fibroblasts.R Soc open sci 2:150402.
• Ferrando L and Scavino AF (2015) Strong shift in the diazotrophic endophytic bacterial community inhabiting rice (Oryza sativa) plants after flooding.FEMS Microbiol Ecol 91:fiv104.
• Miya M, Sato Y, Fukunaga T, et al.(2015) MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species.R Soc open sci 2:150088.
• Zhao C, Jurat-Fuentes JL, Abdelgaffar HM, et al.(2015) Identification of a New cry1I-Type Gene as a Candidate for Gene Pyramiding in Corn To Control Ostrinia Species Larvae.Appl Envir Microbiol 81:3699-3705.
• Martyushev AP, Petravic J, Grimm AJ, et al.(2015) Epitope-Specific CD8+ T Cell Kinetics Rather than Viral Variability Determine the Timing of Immune Escape in Simian Immunodeficiency Virus Infection.J Immunol 194:4112-4121.
• Zhao W, Yang X, Yu H, et al.(2015) RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network.Plant Cell Physiol 56:455-467.
• Stevens S, Cornmell R, Taylor D, et al.(2015) Spatial variations in the microbial community structure and diversity of the human foot is associated with the production of odorous volatiles.FEMS Microbiol Ecol 91:1-11.
• Ericsson AC, Davis DJ, Franklin CL, et al.(2015) Exoelectrogenic capacity of host microbiota predicts lymphocyte recruitment to the gut.Physiol Genomics 47:243-252.
• Urschel MR, Kubo MD, Hoehler TM, et al.(2015) Carbon Source Preference in Chemosynthetic Hot Spring Communities.Appl Envir Microbiol 81:3834-3847.
• Azad AA, Volik SV, Wyatt AW, et al.(2015) Androgen Receptor Gene Aberrations in Circulating Cell-Free DNA: Biomarkers of Therapeutic Resistance in Castration-Resistant Prostate Cancer.Clin Cancer Res 21:2315-2324.
• Krug PW, Holinka LG, O'Donnell V, et al.(2015) The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome.J Virol 89:2324-2332.
• Stasulli NM, Eichelberger KR, Price PA, et al.(2015) Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague.mBio 6:e01530-15.
• Chiarello M, Villéger S, Bouvier C, et al.(2015) High diversity of skin-associated bacterial communities of marine fishes is promoted by their high variability among body parts, individuals and species.FEMS Microbiol Ecol 91:fiv061.
• Metzler-Zebeli BU, Schmitz-Esser S, Mann E, et al.(2015) Adaptation of the Cecal Bacterial Microbiome of Growing Pigs in Response to Resistant Starch Type 4.Appl Envir Microbiol 81:8489-8499.
• Leenen FAD, Vernocchi S, Hunewald OE, et al.(2015) Where does transcription start? 5'-RACE adapted to next-generation sequencing.Nucleic Acids Res 10.1093/nar/gkv1328.
• Wang Q, Holmes N, Martinez E, et al.(2015) It Is Not All about Single Nucleotide Polymorphisms: Comparison of Mobile Genetic Elements and Deletions in Listeria monocytogenes Genomes Links Cases of Hospital-Acquired Listeriosis to the Environmental Source.J Clin Microbiol 53:3492-3500.
• Kapoor V, Pitkänen T, Ryu H, et al.(2015) Distribution of Human-Specific Bacteroidales and Fecal Indicator Bacteria in an Urban Watershed Impacted by Sewage Pollution, Determined Using RNA- and DNA-Based Quantitative PCR Assays.Appl Envir Microbiol 81:91-99.
• Ziels R, Beck DAC, Martí M, et al.(2015) Monitoring the dynamics of syntrophic β-oxidizing bacteria during anaerobic degradation of oleic acid by quantitative PCR.FEMS Microbiol Ecol 91:fiv028.
• Votintseva AA, Pankhurst LJ, Anson LW, et al.(2015) Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) Cultures.J Clin Microbiol 53:1137-1143.
• Jaing CJ, Thissen JB, Gardner SN, et al.(2015) Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs.J Veterinary Diagnostic Investigation 27:313-325.
• Bushell KR, Kim Y, Chan FC, et al.(2015) Genetic inactivation of TRAF3 in canine and human B-cell lymphoma.Blood 125:999-1005.
• Nadimi M, Stefani FOP, Hijri M (2015) The Mitochondrial Genome of the Glomeromycete Rhizophagus sp.  DAOM 213198 Reveals an Unusual Organization Consisting of Two Circular Chromosomes.Genome Biol Evol 7:96-105.
• Plaza DF, Schmieder SS, Lipzen A, et al.(2015) Identification of a Novel Nematotoxic Protein by Challenging the Model Mushroom Coprinopsis cinerea with a Fungivorous Nematode.g3 6:87-98.
• Boothby TC, Tenlen JR, Smith FW, et al.(2015) Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade.PNAS 112:15976-15981.
• Shakya A, Goren A, Shalek A, et al.(2015) Oct1 and OCA-B are selectively required for CD4 memory T cell function.J Exp Med 212:2115-2131.
• Simon JM, Parker JS, Liu F, et al.(2015) A Role for Widely Interspaced Zinc Finger (WIZ) in Retention of the G9a Methyltransferase on Chromatin.J Biol Chem 290:26088-26102.
• Frenel JS, Carreira S, Goodall J, et al.(2015) Serial Next-Generation Sequencing of Circulating Cell-Free DNA Evaluating Tumor Clone Response To Molecularly Targeted Drug Administration.Clin Cancer Res 21:4586-4596.
• Vuong LM, Chellappa K, Dhahbi JM, et al.(2015) Differential Effects of Hepatocyte Nuclear Factor 4α Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells.Mol Cell Biol 35:3471-3490.
• Sharma R, Xia X, Riess K, et al.(2015) Comparative Genomics Including the Early-Diverging Smut Fungus Ceraceosorus bombacis Reveals Signatures of Parallel Evolution within Plant and Animal Pathogens of Fungi and Oomycetes.Genome Biol Evol 7:2781-2798.
• Wong W, An D, Caffrey SM, et al.(2015) Roles of Thermophiles and Fungi in Bitumen Degradation in Mostly Cold Oil Sands Outcrops.Appl Envir Microbiol 81:6825-6838.
• Sawin EA, De Wolfw TJ, Aktas B, et al.(2015) Glycomacropeptide is a prebiotic that reduces Desulfovibrio bacteria, increases cecal short-chain fatty acids, and is anti-inflammatory in mice.Am J Physiol Gastrointest Liver Physiol 309:G590-G601.
• Chaput DL, Hansel CM, Burgos WD, et al.(2015) Profiling Microbial Communities in Manganese Remediation Systems Treating Coal Mine Drainage.Appl Envir Microbiol 81:2189-2198.
• Clemente P, Pajak A, Laine I, et al.(2015) SUV3 helicase is required for correct processing of mitochondrial transcripts.Nucleic Acids Res 43:7398-7413.
• Nagel DH, Doherty CJ, Pruneda-Paz JL, et al.(2015) Genome-wide identification of CCA1 targets uncovers an expanded clock network in Arabidopsis.PNAS 112:E4802-E4810.
• Schneider S, Hendriksen NB, Melin PA, et al.(2015) Chromosome-Directed PCR-Based Detection and Quantification of Bacillus cereus Group Members with Focus on B.  thuringiensis Serovar israelensis Active against Nematoceran Larvae.Appl Envir Microbiol 81:4894-4903.
• Sabat AJ, Pournaras S, Akkerboom V, et al.(2015) Whole-genome analysis of an oxacillin-susceptible CC80 mecA-positive Staphylococcus aureus clinical isolate: insights into the mechanisms of cryptic methicillin resistance.J Antimicrob Chemother 70:2956-2964.
• Wang X, Sharp CE, Jones GM, et al.(2015) Stable-Isotope Probing Identifies Uncultured Planctomycetes as Primary Degraders of a Complex Heteropolysaccharide in Soil.Appl Envir Microbiol 81:4607-4615.
• Eddens T, Elsegeiny W, Nelson MP, et al.(2015) Eosinophils Contribute to Early Clearance of Pneumocystis murina Infection.J Immunol 195:185-193.
• Fernández R and Giribet G. (2015) Unnoticed in the tropics: phylogenomic resolution of the poorly known arachnid order Ricinulei (Arachnida).R Soc open sci 2:150065.
• Prasse CE, Baldwin AH, Yarwood SA, et al.(2015) Site History and Edaphic Features Override the Influence of Plant Species on Microbial Communities in Restored Tidal Freshwater Wetlands.Appl Envir Microbiol 81:3482-3491.
• Bliem R, Schauer S, Plicka H, et al.(2015) A Novel Triplex Quantitative PCR Strategy for Quantification of Toxigenic and Nontoxigenic Vibrio cholerae in Aquatic Environments.Appl Envir Microbiol 81:3077-3085.

• Adams RI, Miletto M, Lindow SE, Taylor JW, Bruns TD (2014) Airborne Bacterial Communities in Residences: Similarities and Differences with Fungi.PLoS ONE 9(3):e91283 doi:10.1371.
  
• Callaway JL, Huang S, Karampetsou E, Crolla JA (2014) Perspective on the technical challenges involved in the implementation of array-CGH in prenatal diagnostic testing.Mol Biotechnol 56(4):312–8.
  
• de Bourcy CFA, De Vlaminck I, Kanbar JN, Wang J, Gawad C, et al.(2014) A Quantitative Comparison of Single-Cell Whole Genome Amplification Methods.PLoS ONE 9(8):e105585. doi:10.1371.
  
• Hewitson L, Thissen JB, Gardner SN, McLoughlin KS, Glausser MK, Jaing CJ, (2014) Screening of Viral Pathogens from Pediatric Ileal Tissue Samples after Vaccination.Advances in Virology, vol. 2014, Article ID 720585 10 pp doi:10.1155.
• Kulkarni AD, Kiron V, Rombout JH et al.(2014) Protein profiling in the gut of Penaeus monodon gavaged with oral WSSV-vaccines and live white spot syndrome virus.Proteomics 14: 1660–1673.
• Sugiyama A, Ueda Y, Zushi T, Takase H, Yazaki K (2014) Changes in the Bacterial Community of Soybean Rhizospheres during Growth in the Field.PLoS ONE 9(6):e100709. doi:10.1371.
  

• Deben C, Zwaenepoel K, Boeckx C et al.(2013) Expression Analysis on Archival Material Revisited: Isolation and Quantification of RNA Extracted From FFPE Samples.Diagn Mol Pathol 22(1):59–64.
• Fitzsimons MS, Novotny M, Lo CC et al.(2013) Nearly finished genomes produced using gel microdroplet culturing reveal substantial intraspecies genomic diversity within the human microbiome.Genome Res 23(5):878–88.
• Hebling CM, McFarland MA, Callahan JH, Ross MM (2013) Global Proteomic Screening of Protein Allergens and Advanced Glycation Endproducts in Thermally Processed Peanuts.J Agric Food Chem 61(24):5638–5648.

• Bundo M, Sunaga F, Ueda J et al.(2012) A systematic evaluation of whole genome amplification of bisulfite-modified DNA.Clin Epigenetics doi:10.1186.
• Gibson WT, Hood RL, Zhan SH et al.(2012) Mutations in EZH2 Cause Weaver Syndrome.Am J Hum Genet 90(1):110–118.
• Kondragunta B, Joshi BH, Han J et al.(2012) Bioreactor environment-sensitive sentinel genes as novel metrics for cell culture scale-down comparability.Biotechnol Prog 28(5):1138–1151.
• Sharp CE, Stott MB, Dunfield PF (2012) Detection of autotrophic verrucomicrobial methanotrophs in a geothermal environment using stable isotope probing.Front Microbiol doi:10.3389.
• Valenzuela J, Mazurie A, Carlson RP et al.(2012) Potential role of multiple carbon fixation pathways during lipid accumulation in Phaeodactylum tricornutum.Biotechnol Biofuels 5(1):40.
• Yang WC, Pate KG, Wong HE, Swartz JR (2012) Simplifying and streamlining Escherichia coli-based cell-free protein synthesis.Biotechnol Prog 28(2):413–420.

• Binder M, Eberle F, Seitz S et al.(2011) Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I).J Biol Chem 286(31):27278–27287.
• Debreuil J, Tambutté S, Zoccola D et al.(2011) Comparative analysis of the soluble organic matrix of axial skeleton and sclerites of Corallium rubrum: Insights for biomineralization.Comp Biochem Physiol B Biochem Mol Biol.Part B 159(1):40–48.
• Ferreira JA, Daniel-da-Silva AL, Alves RM et al.(2011) Synthesis and Optimization of Lectin Functionalized Nanoprobes for the Selective Recovery of Glycoproteins from Human Body Fluids.Anal Chem 83(18):7035–7043.
• Hares K, Kemp K, Gray E et al.(2011) Neurofilament dot blot assays: Novel means of assessing axon viability in culture.J Neurosci Methods 198(2):195–203.
• Holloway, CM, McIntyre CK (2011) Post-training disruption of Arc protein expression in the anterior cingulate cortex impairs long-term memory for inhibitory avoidance training.Neurobiol Learn and Mem 95:425–432.
• Kwezi L, Ruzvidzo O, Wheeler JI et al.(2011) The Phytosulfokine (PSK) Receptor Is Capable of Guanylate Cyclase Activity and Enabling Cyclic GMP-dependent Signaling in Plants.J Biol Chem 286(25):22580–22588.
• Pérez NG, Nolly MB, Roldan MC et al.(2011) Silencing of NHE-1 blunts the slow force response to myocardial stretch.J Appl Physiol 111(3):874–880.
• Recouvreux MV, Guida MC, Rifkin DB et al.(2011) Active and Total Transforming Growth Factor-β1 Are Differentially Regulated by Dopamine and Estradiol in the Pituitary.Endocrinology 152(7):2722–2730.
• Svedružić D, Blackburn JL, Tenent RC et al.(2011) High-Performance Hydrogen Production and Oxidation Electrodes with Hydrogenase Supported on Metallic Single-Wall CarbonNanotube Networks.J Am Chem Sod 133(12):4299–4306.

• Couto GH et al.(2010) Isolation of a novel lipase from a metagenomic library derived from mangrove sediment from the south Brazilian coast.Genetic Mol Res 9(1):514–523.
• Darnell M et al.(2010) Investigation of the involvement of p-glycoprotein and multidrug resistance-associated protein 2 in the efflux of ximelagatran and its metabolites by using short hairpin RNA knockdown Caco-2 cells.Drug Metab Disp 38:491–497.
• Dunn AK et al.(2010) The alternative oxidase (AOX) gene in Vibrio fischeri is controlled aby NsrR and upregulated in response to nitric oxide.Molecular Microbiol 77(1):44–55.
• Fajardo-Cavazos P et al.(2010) Exposure of DNA and Bacillus subtilis Spores to Simulated Martian Environments: Use of Quantitative PCR (qPCR) to Measure Inactivation Rates of DNA to Function as a Template Model.Astrobiology 10(4):403–411.
• Filigheddu N, et al.(2010) Differential Expression of MicroRNAs between Eutopic and Ectopic Endometrium in Ovarian Endometriosis.J Biomed Biotech 2010:369549.
• Gupta RK, Srivastava BS, Srivastava R (2010) Comparative expression analysis of rpf-like genes of Mycobacterium tuberculosis H37Rv under different physiological stress and growth. conditions Microbiology 156:2714–2722.
• Lee, SE et al.(2010) Prevalence of Toxoplasmosis gondii infection in stray and household cats in regions of Seoul, Korea.Korean J Parasitol 48(3):267–270.
• Podolyan, A., et.al.(2010) Identification of the lipoxygenase gene family from Vitis vinifera and biochemical characterization of two 13-lipoxygenases expressed in grape berries of Sauvignon Blanc.Functional Plant Biol 37:767–784.
• Regueiro BJ et.al.(2010) Automated extraction improves multiplex molecular detection of infection in septic patients.PLoS One 5(10):e13387.
• Simmons M, Donovan DM, Siragusa GR, Seal BR (2010) Recombinant Expression of Two Bacteriophage Proteins That Lyse Clostridium perfringens and Share Identical Sequences in the C-Terminal Cell Wall Binding Domain of the Molecules but Are Dissimilar in Their N-Terminal Active Domains.J Agric Food Chem 58(19):10330–10337.
• Tennico YH et.al.(2010) On-chip aptamer-based sandwich assay for thrombin detection employing magnetic beads and quantum dots.Anal Chem 82:5591–5597.

• D'Andrea M, Coïsson JD, Travaglia F et al.(2009) Development and Validation of a SYBR-Green I Real-Time PCR Protocol To Detect Hazelnut (Corylus avellana L.) in Foods through Calibration via Plasmid Reference Standard.J Agric Food Chem 57(23):11201–11208.