小鼠 T 细胞活化与扩增

即用型 Dynabeads 可同步向 TCR/CD3 和 CD28 进行信号转导，使小鼠 T 细胞进行生理活化和扩增。 这项技术超越了传统自制活化方法（有丝分裂原， ConA ，可溶性抗体等），并在 已发表的文献中有详细记载。

Dynabeads 允许您使用相同的技术平台进行活化/扩增，将小鼠研究转向临床研究。

为在 T 细胞活化前分离 T 细胞，我们提供一系列用于阳性和阴性分离的 Dynabeads。查看 选择指南

以下列出的参考文献是众多已发表论文中的一部分，这些论文记录了 Dynabeads 技术在 T 细胞活化和扩增方面的表现：

1. He, J el al.(2016) Low-dose interleukin-2 treatment selectively modulates CD4⁺ T cell subsets in patients with systemic lupus erythematosus.Nature Medicine 22: 991-993.
2. Yamada, A et al.(2015) Impaired expansion of regulatory T cells in a neonatal thymectomy-induced autoimmune mouse model.Am J Pathology 185: 2886-97.
3. Keenan, BP et al.(2014) A Listeria vaccine and depletion of T-regulatory cells activate immunity against early stage pancreatic intraepithelial neoplasms and prolong survival of mice.Gastroenterology 146: 1784-1796.
4. Jhunjhunwala, S et al.(2012) Controlled release formulations of IL-2, TGF-β1 and rapamycin for the induction of regulatory T cells.J Control Release 159: 78-84.
5. Rapoport, A.P. et al.(2005) Restoration of immunity in lymphopenic individuals with cancer by vaccination and adoptive T-cell transfer.Nat Med 11:1162–1163.
6. Bonyhadi, M. et al.(2005) In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia.J Immunol 174:2366–2375.
7. Godfrey, W.R. et al.(2004) In vitro expanded human CD4+CD25+ T regulatory cells can markedly inhibit allogeneic dendritic cell stimulated MLR cultures.Blood 104:453–461.
8. Coito, S. et al.(2004) Retrovirus-mediated gene transfer in human primary T lymphocytes induces an activation-and transduction/selection-dependent TCRBV repertoire skewing of gene-modified cells.Stem Cells Dev 13:71–81.
9. Hami, L. et al.(2003) Comparison of a static process and a bioreactor-based process for the GMP manufacture of autologous Xcellerated T cells for clinical trials.BioProcessing Journal Vol. 2 No. 6, November/December 2003.
10. Berger, C. et al.(2003) CD28 costimulation and immunoaffinity-based selection efficiently generate primary gene-modified T cells for adoptive immunotherapy.Blood 101:476–484.
11. Levine, B.L. et al.(2002) Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreases CCR5 expression in HIV infection.Nat Med 8:47–53.