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产品规格
检测灵敏度
4 pg/mL
测定范围
4-500 pg/mL
检测样本类型/体积
{Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilutions of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilutions of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-15/IL-15R
均相（无需洗涤）
Mouse
检测试剂盒格式
Uncoated ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Mouse Interleukin 15/IL-15 Receptor Complex (IL-15/IL-15R) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-15/IL-15R. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AA690181, AI503618, IL-15, IL-15RA, Il15, Il15ra
基因ID : (Mouse) 16168, (Mouse) 16169
基因符号 : Il15, Il15ra
蛋白别名 : CD215, IL-15, IL-15 receptor subunit alpha, IL-15R-alpha, ILN, Interleukin, Interleukin-15, Interleukin-15 receptor subunit alpha, sIL-15 receptor subunit alpha, Soluble interleukin-15 receptor subunit alpha
UniProt ID (Mouse) P48346, (Mouse) Q60819
