Invitrogen

CD8a Monoclonal Antibody (53-6.7), eFluor™ 615, eBioscience™

CD8a Antibody in Immunohistochemistry (Frozen) (IHC (F))

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CD8a Antibody (42-0081-82) in IHC (F)

Immunohistochemistry on frozen mouse spleen using 5 µg/mL Rat IgG2a Isotype Control eFluor® 615 (left) or 5 µg/mL Anti-Mouse CD8a eFluor® 615 (right). Nuclei are counterstained with DAPI.

CD8a Antibody in Immunocytochemistry (ICC/IF)

CD8a Antibody in Immunohistochemistry (IHC)

CD8a Antibody in Immunoprecipitation (IP)

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产品信息

42-0081-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看17篇已发表文章 17篇已发表文章

免疫组化（冰冻） (IHC (F))

5 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

查看7篇已发表文章 7篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

53-6.7

偶联物

eFluor™ 615 eFluor™ 615 eFluor™ 615

激发/发射光谱

595/614 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10805750

产品详细信息

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported: This 53-6.7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunocytochemistry.

Applications Tested: This 53-6.7 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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An antigen processing and presentation signature for prognostic evaluation and immunotherapy selection in advanced gastric cancer.

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Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

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Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression.

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42-0081-82 was used in Immunohistochemistry to investigate codon optimised gene therapies in alleviating side effects for future treatments.

Mon Jan 17 00:00:00 UTC 2022

Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression.

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Starikova AV,Skopenkova VV,Polikarpova AV,Reshetov DA,Vassilieva SG,Velyaev OA,Shmidt AA,Savchenko IM,Soldatov VO,Egorova TV,Bardina MV

42-0081-82 was used in Immunohistochemistry to investigate codon optimised gene therapies in alleviating side effects for future treatments.

Mon Jan 17 00:00:00 UTC 2022

A Small Molecule Antagonist of PD-1/PD-L1 Interactions Acts as an Immune Checkpoint Inhibitor for NSCLC and Melanoma Immunotherapy.

Frontiers in immunology

Wang Y,Gu T,Tian X,Li W,Zhao R,Yang W,Gao Q,Li T,Shim JH,Zhang C,Liu K,Lee MH

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

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A Cu₉S₅ nanoparticle-based CpG delivery system for synergistic photothermal-, photodynamic- and immunotherapy.

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Zhou L,Chen L,Hu X,Lu Y,Liu W,Sun Y,Yao T,Dong C,Shi S

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Fri Jul 03 00:00:00 UTC 2020

Stroma remodeling and reduced cell division define durable response to PD-1 blockade in melanoma.

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Galvani E,Mundra PA,Valpione S,Garcia-Martinez P,Smith M,Greenall J,Thakur R,Helmink B,Andrews MC,Boon L,Chester C,Gremel G,Hogan K,Mandal A,Zeng K,Banyard A,Ashton G,Cook M,Lorigan P,Wargo JA,Dhomen N,Marais R

42-0081-82 was used in Immunohistochemistry to show the genetic and phenotypic changes induced by treatment with programmed cell death-1 (PD-1) blockade in a genetically engineered mouse model of melanoma driven by oncogenic BRAF.

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NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer.

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42-0081-82 was used in Immunocytochemistry to verify that triple-negative breast cancer (TNBC) has higher PD-L1 expression than other subtypes.

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Hemozoin produced by mammals confers heme tolerance.

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Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

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Type 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis.

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The inflammatory phase of fracture healing is influenced by oestrogen status in mice.

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Adeno-associated virus serotype rh.10 displays strong muscle tropism following intraperitoneal delivery.

Scientific reports

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Mon Jan 09 00:00:00 UTC 2017

Metabolic maintenance of cell asymmetry following division in activated T lymphocytes.

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Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33.

Scientific reports

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Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Tue Sep 13 00:00:00 UTC 2016

MHC-I and PirB Upregulation in the Central and Peripheral Nervous System following Sciatic Nerve Injury.

PloS one

Bombeiro AL,Thomé R,Oliveira Nunes SL,Monteiro Moreira B,Verinaud L,Oliveira AL

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Wed Jul 26 00:00:00 UTC 2017

Postsynaptic SDC2 induces transsynaptic signaling via FGF22 for bidirectional synaptic formation.

Scientific reports

Hu HT,Umemori H,Hsueh YP

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunoprecipitation

Thu Sep 15 00:00:00 UTC 2016

CD31 signals confer immune privilege to the vascular endothelium.

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Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Tue Oct 27 00:00:00 UTC 2015

Imaging Tolerance Induction in the Classic Medawar Neonatal Mouse Model: Active Roles of Multiple F1-Donor Cell Types.

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Huang HN,Chan YL,Hui CF,Wu JL,Wu CJ,Chen JY

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Sat May 30 00:00:00 UTC 2015

Adjuvant cationic liposomes presenting MPL and IL-12 induce cell death, suppress tumor growth, and alter the cellular phenotype of tumors in a murine model of breast cancer.

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42-0081 was used in Immunohistochemistry to show how combination liposomal therapy was able to induce similar reductions in tumor growth in both treated and distal tumors, suggesting a systemic immune response.

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Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Western Blot

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In vivo CD8+ T cell dynamics in the liver of Plasmodium yoelii immunized and infected mice.

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Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

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A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific manner.

PloS one

Schuster C,Brosi H,Stifter K,Boehm BO,Schirmbeck R

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Tue Apr 08 00:00:00 UTC 2014

Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.

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生物信息学

蛋白别名: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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基因别名: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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功能

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

参与通路

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus