Induced Pluripotent Stem Cell (iPSC) Media and Reagents
for Cell Therapy

First off-the-shelf reprogramming system manufactured in accordance with GMP requirements. The Invitrogen CTS CytoTune 2.1 kit offers high-efficiency Sendai delivery of reprogramming factors.

• Xeno-free workflow—generate iPSC lines from both fibroblasts and blood for clinical research
• Regulatory submission support—documentation such as a regulatory support file to support regulatory submission
• Designed with transition in mind—complimentary research-use CytoTune iPS 2.0 Sendai Reprogramming Kit helps facilitate a seamless translation to the clinic

Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the only globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals.

• Helps reduce risk—fully defined
• Enables seamless transition—same fully defined and highly consistent eight-component formulation as RUO Essential 8 Medium but with animal origin–free components
• Supports long-term iPSC culture—maintains iPSC marker expression, trilineage differentiation potential, and a normal karyotype over multiple passages

Gibco CTS Essential 6 Medium is a xeno-free, feeder-free, cGMP-manufactured medium which supports the spontaneous or directed differentiation of human pluripotent stem cells (PSCs) and the reprogramming of somatic cells.

• Provides a flexible format—suits different applications and various cell types
• Simplifies the transition from bench to clinic—contains the same six essential components as RUO Essential 6 Medium, and with its cGMP manufacturing process, this formulation helps minimize variability

Gibco CTS Vitronectin (VTN-N) Recombinant Human Protein is a defined matrix for feeder-free culture of iPSCs.Designed in the laboratory of James Thomson, this recombinant protein is intended for use with the CTS Essential 8 culture system.

• Animal origin–free—microbial expression recombinant matrix helps reduce variability and viral contamination risk in iPSC cultures
• Optimized—VTN-N variant has been shown to support human iPSC attachment and survival better than wild type vitronectin
• Supports pluripotency—able to maintain pluripotency, normal growth characteristics, trilineage potential and normal karyotype after extended culture

Gibco CTS RevitaCell Supplement (100X) is an animal-origin-free, chemically defined supplement used with PSCs for post-thaw recovery or in combination with CTS Essential 8 Medium for single cell passaging. To minimize both the loss of cell viability and differentiation of PSCs, use the CTS PSC Cryopreservation Kit.

• Improved cell survival—provides high cell viability post-single cell passaging and post-thaw
• Contains higher specificity inhibitor—includes a higher specificity ROCK inhibitor, coupled with antioxidants and free radical scavengers to minimize impact of stress on cells
• Simplifies transition to clinic—animal-origin-free formulation

Gibco CTS Versene is a gentle non-enzymatic cell dissociation reagent for use in routine clump passaging of PSCs while maintaining viability over multiple passages.

• Designed for cell therapy applications—manufactured using methods and controls that conform to cGMP for medical devices, 21 CFR Part 820
• Seamless transition from bench to clinic—same formulation as RUO Versene Solution but with additional characterization and quality control testing

For the cryopreservation and recovery of PSCs, the Gibco CTS PSC Cryopreservation Medium and CTS RevitaCell Supplement minimize the loss of cell viability and maximize post-thaw recovery when used in combination. Both reagents are included in the CTS PSC Cryopreservation Kit.

• Provides efficient recovery—routinely achieves higher viability than traditional methods of cryopreservation
• Offers consistency—chemically defined media maintains normal morphology, pluripotency, and karyotype of PSCs
• Reduces variability—optimized reagents minimize the causes of genetic instability of PSC

Gibco CTS PSC Cryopreservation Medium is a xeno-free solution for the cryopreservation of pluripotent stem cells (PSCs). Both CTS PSC Cryopreservation Medium and CTS RevitaCell supplement are included in the CTS PSC Cryopreservation Kit that helps minimize loss of cell viability and maximize post-thaw recovery.

• Delivers higher recovery efficiency—efficient recovery and higher viability than traditional methods of cryopreservation
• Provides versatility—compatible with clump and singe cell passaged PSCs cultured in a variety of media systems
• Offers ease of use—provided as a ready-to-use , complete solution with no required additions of enhancing reagents

Gibco CTS KnockOut SR XenoFree Medium is a defined, xeno-free serum replacement based on the traditional KnockOut Serum Replacement, which has been cited in more than 2,000 publications and trusted for over 20 years.

• Supports pluripotency—able to maintain the pluripotency, normal morphology, and karyotype of hPSCs
• Versatile—can also be used for cryopreservation, derivation, and differentiation of iPSCs
• Designed for your applications—a first-to-market, xeno-free iPSC product

Your choice of chemically defined human- and animal origin-free basal media for pluripotent stem cell culture. Based on traditional DMEM and DMEM/F12 formulations, these basal media are:

• Optimized—lower osmolarity compared vs. traditional formulations improve maintenance of undifferentiated iPSCs
• Versatile—have been shown to support expansion and differentiation of a variety of stem cells aside from iPSCs
• Tried and tested—cited in many publications, often used with CTS KnockOut SR XenoFree Medium