Invitrogen

TCR beta Monoclonal Antibody (H57-597), Biotin, eBioscience™

TCR beta Antibody in Flow Cytometry (Flow)

图: 1 / 35

TCR beta Antibody (13-5961-82) in Flow

Staining of mouse splenocytes with Anti-Mouse TCR beta FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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产品信息

13-5961-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看31篇已发表文章 31篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

H57-597

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_466819

产品详细信息

Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.

Applications Reported: The H57-597 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The H57-597 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Mon Apr 05 00:00:00 UTC 2021

Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.

Immunity

Soni C,Perez OA,Voss WN,Pucella JN,Serpas L,Mehl J,Ching KL,Goike J,Georgiou G,Ippolito GC,Sisirak V,Reizis B

13-5961-82 was used in Flow Cytometry to establish extrafollicular B cell differentiation into short-lived AFCs as a key mechanism of anti-DNA autoreactivity and reveal a major contribution of pDCs, endosomal Toll-like receptors (TLRs), and IFN-I to this pathway.

Tue Jun 16 00:00:00 UTC 2020

Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development.

The Journal of cell biology

Romero-Wolf M,Shin B,Zhou W,Koizumi M,Rothenberg EV,Hosokawa H

13-5961 was used in Flow cytometry/Cell sorting to define a distinct mechanism of Notch signal response that distinguishes the initial stages of murine T cell development.

Mon Oct 05 00:00:00 UTC 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Mon May 04 00:00:00 UTC 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Tue Dec 08 00:00:00 UTC 2020

Critical role of WNK1 in MYC-dependent early mouse thymocyte development.

eLife

Köchl R,Vanes L,Llorian Sopena M,Chakravarty P,Hartweger H,Fountain K,White A,Cowan J,Anderson G,Tybulewicz VL

13-5961 was used in Flow cytometry/Cell sorting to ind that WNK1 is required for the transition of double negative (DN) thymocytes through the β-selection checkpoint and subsequent proliferation and differentiation into double positive (DP) thymocytes.

Wed Oct 14 00:00:00 UTC 2020

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Mon Jul 06 00:00:00 UTC 2020

Use of Busulfan to Condition Mice for Bone Marrow Transplantation.

STAR protocols

Montecino-Rodriguez E,Dorshkind K

13-5961 was used in Flow cytometry/Cell sorting to describe a protocol for the use of busulfan to condition mice for bone marrow transplantation and several of the variables to consider for effective implementation.

Fri Dec 18 00:00:00 UTC 2020

CD160 serves as a negative regulator of NKT cells in acute hepatic injury.

Nature communications

Kim TJ,Park G,Kim J,Lim SA,Kim J,Im K,Shin MH,Fu YX,Del Rio ML,Rodriguez-Barbosa JI,Yee C,Suh KS,Kim SJ,Ha SJ,Lee KM

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Mon Jul 22 00:00:00 UTC 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Thu Jan 25 00:00:00 UTC 2018

Interleukin-17-Producing γδ T Cells Originate from SOX13⁺ Progenitors that Are Independent of γδTCR Signaling.

Immunity

Spidale NA,Sylvia K,Narayan K,Miu B,Frascoli M,Melichar HJ,Zhihao W,Kisielow J,Palin A,Serwold T,Love P,Kobayashi M,Yoshimoto M,Jain N,Kang J

13-5961-82 was used in Flow Cytometry to generate reporter mice for the Tγδ17 lineage-defining transcription factor SOX13 and identify fetal-origin, intrathymic Sox13+ progenitors.

Tue Nov 20 00:00:00 UTC 2018

STAT1 is a sex-specific tumor suppressor in colitis-associated colorectal cancer.

Molecular oncology

Crnčec I,Modak M,Gordziel C,Svinka J,Scharf I,Moritsch S,Pathria P,Schlederer M,Kenner L,Timelthaler G,Müller M,Strobl B,Casanova E,Bayer E,Mohr T,Stöckl J,Friedrich K,Eferl R

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Thu Sep 06 00:00:00 UTC 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

13-5961-82 was used in Flow Cytometry to study the activation of Bcl11b, which controls T-cell fate commitment.

Tue Nov 20 00:00:00 UTC 2018

A Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic Leukemia.

Cancer cell

Robles-Valero J,Lorenzo-Martín LF,Menacho-Márquez M,Fernández-Pisonero I,Abad A,Camós M,Toribio ML,Espinosa L,Bigas A,Bustelo XR

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Mon Nov 13 00:00:00 UTC 2017

1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8⁺ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model.

Frontiers in immunology

Dai K,Huang L,Huang YB,Chen ZB,Yang LH,Jiang YA

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

Targeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model.

Scientific reports

Alzubi J,Pallant C,Mussolino C,Howe SJ,Thrasher AJ,Cathomen T

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Fri Sep 29 00:00:00 UTC 2017

NLRP2 controls age-associated maternal fertility.

The Journal of experimental medicine

Kuchmiy AA,D'Hont J,Hochepied T,Lamkanfi M

13-5961 was used in Flow cytometry/Cell sorting to identify Nlrp2 as a critical regulator of oocyte quality.

Mon Dec 12 00:00:00 UTC 2016

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2016

Environmental and Genetic Activation of Hypothalamic BDNF Modulates T-cell Immunity to Exert an Anticancer Phenotype.

Cancer immunology research

Xiao R,Bergin SM,Huang W,Slater AM,Liu X,Judd RT,Lin ED,Widstrom KJ,Scoville SD,Yu J,Caligiuri MA,Cao L

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Wed Jun 01 00:00:00 UTC 2016

Protein kinase D regulates positive selection of CD4⁺ thymocytes through phosphorylation of SHP-1.

Nature communications

Ishikawa E,Kosako H,Yasuda T,Ohmuraya M,Araki K,Kurosaki T,Saito T,Yamasaki S

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Tue Sep 27 00:00:00 UTC 2016

Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.

Nature communications

Meininger I,Griesbach RA,Hu D,Gehring T,Seeholzer T,Bertossi A,Kranich J,Oeckinghaus A,Eitelhuber AC,Greczmiel U,Gewies A,Schmidt-Supprian M,Ruland J,Brocker T,Heissmeyer V,Heyd F,Krappmann D

13-5961 was used in Flow cytometry/Cell sorting to study how TCR-induced alternative splicing augments MALT1 scaffolding to enhance downstream signalling and to promote optimal T-cell activation.

Tue Apr 12 00:00:00 UTC 2016

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Tue Dec 29 00:00:00 UTC 2015

Antibiotics in neonatal life increase murine susceptibility to experimental psoriasis.

Nature communications

Zanvit P,Konkel JE,Jiao X,Kasagi S,Zhang D,Wu R,Chia C,Ajami NJ,Smith DP,Petrosino JF,Abbatiello B,Nakatsukasa H,Chen Q,Belkaid Y,Chen ZJ,Chen W

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Tue Sep 29 00:00:00 UTC 2015

Regulatory T cells promote a protective Th17-associated immune response to intestinal bacterial infection with C. rodentium.

Mucosal immunology

Wang Z,Friedrich C,Hagemann SC,Korte WH,Goharani N,Cording S,Eberl G,Sparwasser T,Lochner M

13-5961 was used in Flow cytometry/Cell sorting to study the impact of Foxp3(+) Tregs on inflammatory processes elicited by the bacterial pathogen C. rodentium, showing that Tregs promote a protective Th17-associated immune response.

Sat Nov 01 00:00:00 UTC 2014

Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

Nature immunology

Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

Published figure using TCR beta monoclonal antibody (Product # 13-5961-82) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2013

Wnt4 enhances murine hematopoietic progenitor cell expansion through a planar cell polarity-like pathway.

PloS one

Heinonen KM,Vanegas JR,Lew D,Krosl J,Perreault C

13-5961 was used in Flow cytometry/Cell sorting to establish a functional role for non-canonical Wnt signalling in hematopoiesis through a pathway involving Wnt4, Fz6, Rac1 and Jnk kinases.

Tue Apr 26 00:00:00 UTC 2011

Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production.

Blood

Qi Q,Xia M,Hu J,Hicks E,Iyer A,Xiong N,August A

13-5961 was used in Flow cytometry/Cell sorting to investigate the effect of Itk on T cell populations, showing that development of CD4+ gammadelta T cells is enhanced and IgE production is elevated in the absence of Itk.

Thu Jul 16 00:00:00 UTC 2009

Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.

The Journal of experimental medicine

Gräbner R,Lötzer K,Döpping S,Hildner M,Radke D,Beer M,Spanbroek R,Lippert B,Reardon CA,Getz GS,Fu YX,Hehlgans T,Mebius RE,van der Wall M,Kruspe D,Englert C,Lovas A,Hu D,Randolph GJ,Weih F,Habenicht AJ

13-5961 was used in Flow cytometry/Cell sorting to investigate how the LTbetaR pathway has a major role in shaping the immunological characteristics and overall integrity of the arterial wall.

Fri Jan 16 00:00:00 UTC 2009

Sequential phases in the development of Aire-expressing medullary thymic epithelial cells involve distinct cellular input.

European journal of immunology

White AJ,Withers DR,Parnell SM,Scott HS,Finke D,Lane PJ,Jenkinson EJ,Anderson G

13-5961 was used in Flow cytometry/Cell sorting to analyse the cellular and molecular requirements for the initial appearance of Aire+ medullary thymic epithelial cells in the embryonic thymus, in addition to their persistence in the adult thymus.

Tue Apr 01 00:00:00 UTC 2008

Hierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation.

The Journal of experimental medicine

Besseyrias V,Fiorini E,Strobl LJ,Zimber-Strobl U,Dumortier A,Koch U,Arcangeli ML,Ezine S,Macdonald HR,Radtke F

13-5961 was used in Flow cytometry/Cell sorting to study the Notch-Delta interactions that promote T cell development.

Mon Feb 19 00:00:00 UTC 2007

生物信息学

蛋白别名: bTCR; FLJ22602; MGC117436; MGC22624; MGC23964; MGC71411; t-cell antigen receptor; T-cell receptor; TCR beta; Tcra; TCRbeta; TCRD; TRA

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基因别名: TCRbeta; Tib

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Entrez Gene ID: (Mouse) 21577

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TCR beta Monoclonal Antibody (H57-597), Biotin, eBioscience™

TCR beta Antibody (13-5961-82) in Flow

产品信息

13-5961-82

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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