Invitrogen

TCR beta Monoclonal Antibody (H57-597), eFluor™ 450, eBioscience™

TCR beta Antibody in Flow Cytometry (Flow)

图: 1 / 35

TCR beta Antibody (48-5961-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD4 APC (Product # 17-0041-82) and 0.125 µg of Armenian Hamster IgG Isotype Control eFluor® 450 (Product # 48-4888-82) (left) or 0.125 µg of Anti-Mouse TCR beta eFluor® 450 (right). Total viable cells were used for analysis.

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产品信息

48-5961-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看37篇已发表文章 37篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

H57-597

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11039532

产品详细信息

Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.

Applications Reported: This H57-597 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H57-597 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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48-5961-82 was used in Flow cytometry/Cell sorting to reveal an important role for CD82 in restraining phagocyte infiltration and mediating their differentiation in response to stimulatory cues.

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48-5961-82 was used in Flow cytometry/Cell sorting to explore endogenous in vivo Treg expansion through antibody-mediated agonistic stimulation of the tumor necrosis factor receptor superfamily member 25 (TNFRSF25) pathway and its potential to prolong graft survival in a mouse model of islet allotransplantation.

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48-5961-82 was used in Flow cytometry/Cell sorting to demonstrate spatiotemporal differences in the production and action of IL-22 by ILCs and T cells during infection and reveal an indispensable role for IL-22-producing T cells in the protection of the intestinal crypts.

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48-5961-82 was used in Flow cytometry/Cell sorting to highlight a distinct metabolic requirement of NKT cells compared with CD4 T cells, which may have therapeutic implications in the treatment of certain nutrient-restricted diseases.

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48-5961 was used in Flow cytometry/Cell sorting to provide demonstration of DA-DRD5 signaling in colonic macrophages controlling the development of colitis by regulating M1/M2 macrophage polarization.

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Published figure using TCR beta monoclonal antibody (Product # 48-5961-82) in Flow Cytometry

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Antibiotics in neonatal life increase murine susceptibility to experimental psoriasis.

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Zanvit P,Konkel JE,Jiao X,Kasagi S,Zhang D,Wu R,Chia C,Ajami NJ,Smith DP,Petrosino JF,Abbatiello B,Nakatsukasa H,Chen Q,Belkaid Y,Chen ZJ,Chen W

Published figure using TCR beta monoclonal antibody (Product # 48-5961-82) in Flow Cytometry

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Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

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Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

Published figure using TCR beta monoclonal antibody (Product # 48-5961-82) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2013

生物信息学

蛋白别名: bTCR; FLJ22602; MGC117436; MGC22624; MGC23964; MGC71411; t-cell antigen receptor; T-cell receptor; TCR beta; Tcra; TCRbeta; TCRD; TRA

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基因别名: TCRbeta; Tib

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Entrez Gene ID: (Mouse) 21577

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TCR beta Monoclonal Antibody (H57-597), eFluor™ 450, eBioscience™

TCR beta Antibody (48-5961-82) in Flow

产品信息

48-5961-82

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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